Overview

  • Product name

    Anti-Uroplakin III antibody [SFI-1], prediluted
    See all Uroplakin III primary antibodies
  • Description

    Mouse monoclonal [SFI-1] to Uroplakin III, prediluted
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to an internal region of Human Uroplakin III.

  • Positive control

    • Urothelial tissue, bladder.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituents: buffer with carrier protein and preservative.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    SFI-1
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Ready to use for 30 min at RT.

 

IHC-Fr Use at an assay dependent concentration. PubMed: 21055807
WB Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.

Target

  • Function

    Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence.
  • Tissue specificity

    Expressed in ureter.
  • Involvement in disease

    Defects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy.
  • Sequence similarities

    Belongs to the uroplakin-3 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER).
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC119178 antibody
    • OTTHUMP00000028951 antibody
    • UP III antibody
    • UP3a antibody
    • UPIII antibody
    • UPIIIA antibody
    • UPK 3 antibody
    • UPK 3A antibody
    • UPK3 antibody
    • UPK3A antibody
    • UPK3A_HUMAN antibody
    • Uroplakin 3A antibody
    • Uroplakin III antibody
    • Uroplakin-3a antibody
    • Uroplakin3A antibody
    • UroplakinIII antibody
    see all

Images

  • ab78197 staining Uroplakin III in human bladder by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • ab78197 staining Uroplakin III in mouse bladder tissue sections. Tissue was fixed in formaldehyde and heat-mediated antigen retrieval was performed using citric buffer. Blocking was performed with 1% BSA for 10 minutes at 21ºC and ab78197 was used at 1/30 for 2 hours at 21ºC.

    See Abreview

  • ab78197 staining Uroplakin III in rat bladder tissue sections. Tissue was fixed in formaldehyde and heat-mediated antigen retrieval was performed using citric buffer. Blocking was performed with 1% BSA for 10 minutes at 21ºC and ab78197 was used at 1/30 for 2 hours at 21ºC.

    See Abreview

References

ab78197 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Urinary bladder)
Specification
Urinary bladder
Permeabilization
No
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 10 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Urinary bladder)
Specification
Urinary bladder
Permeabilization
No
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 10 2014

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