Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Marmoset (common) Tissue sections (Bladder)
Specification
Bladder
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250
Additional data
Additional Notes
Submitted image of urothelium shows only non-specific nuclear positivity
Abcam response
Thank you for your valuable feedback. ab93721 has not been previously tested in marmoset tissue and your data suggests that ab93721 may not be suitable for detecting Uroplakin III on marmoset tissue using IHC-P under these experimental conditions. We hope this information is useful to our customers.
Mr. Carl Hobbs
Verified customer
Submitted Dec 06 2013