Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Uroplakin III antibody [SP73]

Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Bladder)
Specification
Bladder
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
Submitted image shows poor labelling of Uroplakin. Occasional nuclei are positive.
Abcam response
Thank you for your valuable feedback. ab93721 has not been previously tested in rat tissue and your data suggests that ab93721 may not be suitable for detecting Uroplakin III on rat tissue using IHC-P under these experimental conditions. We hope this information is useful to our customers.

Mr. Carl Hobbs

Verified customer

Submitted Dec 05 2013

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