Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Uroplakin III antibody [SP73]

Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Bladder)
Specification
Bladder
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
Submitted image shows all of the Urothelium as positive ( excepting the lumenal aspect of the Umbrella cells which appears very pale).
Abcam response
Many thanks for your valuable feedback. Abcam has not validated the species used in this Abreview. It appears that under these experimental conditions, ab93721 does not specifically detect Uroplakin III in mouse. We welcome further customer feedback. For more information, please contact scientific support.

Mr. Carl Hobbs

Verified customer

Submitted Aug 07 2014

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