Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Uroplakin III antibody [SP73]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Ureter)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Ureter
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/50

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Submitted composite image shows appropriate positivity in the lumenal cytoplasm of the surface cells ( upper image- albeit one can also see a slight nuclear positivity). However, as in cow, there is extensive kidney positivity ( lower image).
Abcam response
Thank you for your valuable feedback. ab93721 has not been previously tested in goat tissue. There is no sequence homology between the immunogen sequence and a goat protein. Your data demonstrates that ab93721 is not suitable for detecting Uroplakin III on goat tissue using IHC-P under these experimental conditions. We hope this information is useful to our customers.

Mr. Carl Hobbs

Verified customer

Submitted Nov 30 2015

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