Recombinant
RabMAb

Recombinant Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)

Overview

  • Product name

    Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free
    See all Uroplakin II/UPII primary antibodies
  • Description

    Rabbit monoclonal [EPR18799] to Uroplakin II/UPII - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: O00526

  • Positive control

    • IHC-P: Human bladder, ureter and bladder cancer tissues.
  • General notes

    ab224261 is the carrier-free version of ab213655 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab224261 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Uroplakin II.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18799
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in regulating the assembly of the AUM.
  • Cellular localization

    Cell membrane
  • Database links

  • Alternative names

    • MGC138598 antibody
    • UP2 antibody
    • UPII antibody
    • UPK2 antibody
    • Uroplakin 2 antibody
    • Uroplakin II antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human ureter tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and weak membrane staining on epithelial cells of human ureter tissues is observed [PMID:24978921]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213655).

  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on tumor cells of human bladder cancer is observed [PMID:24978921]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213655).

  • Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on tumor cells of human renal clear cell carcinoma. [PMID:24978921].

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213655).

  • This IHC data was generated using the same anti-Uroplakin II/UPII antibody clone [EPR18799] in a different buffer formulation (cat# ab213655).

    Immunohistochemical analysis of paraffin-embedded human bladder tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on epithelial cells of human bladder tissues is observed [PMID:24978921]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab224261 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224261.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up