Key features and details
- Mouse monoclonal [3D6] to URP2/Kindlin-3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-URP2/Kindlin-3 antibody [3D6]
See all URP2/Kindlin-3 primary antibodies
DescriptionMouse monoclonal [3D6] to URP2/Kindlin-3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human URP2/Kindlin-3. Bacterial expressed.
EpitopeThe epitope for clone 3D6 has been mapped to residues 181-461.
- This antibody gave a positive signal in human spleen tissue lysate and in the following whole cell lysates: Raji; K562; THP1. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal spleen.
This antibody clone is manufactured by Abcam.
Previously labelled as URP2.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab173416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).|
|IHC-P||Use a concentration of 0.1 µg/ml.|
FunctionPlays a central role in cell adhesion in hematopoietic cells. Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells. Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs).
Isoform 2 may act as a repressor of NF-kappa-B and apoptosis.
Tissue specificityHighly expressed in lymph node. Expressed in thymus, spleen and leukocytes. Weakly expressed in placenta, small intestine, stomach, testis and lung. Overexpressed in B-cell malignancies.
Involvement in diseaseDefects in FERMT3 are the cause of leukocyte adhesion deficiency type 3 (LAD3) [MIM:612840]; also called leukocyte adhesion deficiency 1 variant (LAD1v). LAD3 is a rare syndrome characterized by infections without pus formation in the presence of a leukocytosis combined with a Glanzmann-type bleeding disorder, resulting from a hematopoietic defect in integrin activation. Symptoms arise from an inability to activate the integrins expressed on hematopoietic cells, including platelets and leukocytes.
Sequence similaritiesBelongs to the kindlin family.
Contains 1 FERM domain.
Contains 1 PH domain.
DomainThe FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
Cellular localizationCell projection > podosome. Present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells.
- Information by UniProt
- Fermitin family homolog 3 antibody
- Fermitin family member 3 antibody
- FERMT3 antibody
IHC image of URP2/Kindlin-3 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab173416, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-URP2/Kindlin-3 antibody [3D6] (ab173416) at 5 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 4 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 33 kDa (possible cleavage fragment)
Exposure time: 4 minutes
We believe that the band observed at approximately 33-kDa is a C-terminal cleavage fragment of URP2/Kindlin-3, which has been described in the literature (PMID 23012377). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab173416 has been referenced in 2 publications.
- Feng C et al. Expression of kindlin-3 in melanoma cells impedes cell migration and metastasis. Cell Adh Migr 11:419-433 (2017). PubMed: 27715393
- Qu J et al. Kindlin-3 interacts with the ribosome and regulates c-Myc expression required for proliferation of chronic myeloid leukemia cells. Sci Rep 5:18491 (2015). IP, ICC/IF ; Human . PubMed: 26677948