Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)

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Overview

  • Product name

    Anti-URP2/Kindlin-3 antibody [3D6]
    See all URP2/Kindlin-3 primary antibodies

  • Description

    Mouse monoclonal [3D6] to URP2/Kindlin-3

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein corresponding to Human URP2/Kindlin-3. Bacterial expressed.

  • Epitope

    The epitope for clone 3D6 has been mapped to residues 181-461.

  • Positive control

    • This antibody gave a positive signal in human spleen tissue lysate and in the following whole cell lysates: Raji; K562; THP1. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal spleen.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Previously labelled as URP2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab173416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
IHC-P Use a concentration of 0.1 µg/ml.

Target

  • Function

    Plays a central role in cell adhesion in hematopoietic cells. Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells. Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs).
    Isoform 2 may act as a repressor of NF-kappa-B and apoptosis.

  • Tissue specificity

    Highly expressed in lymph node. Expressed in thymus, spleen and leukocytes. Weakly expressed in placenta, small intestine, stomach, testis and lung. Overexpressed in B-cell malignancies.

  • Involvement in disease

    Defects in FERMT3 are the cause of leukocyte adhesion deficiency type 3 (LAD3) [MIM:612840]; also called leukocyte adhesion deficiency 1 variant (LAD1v). LAD3 is a rare syndrome characterized by infections without pus formation in the presence of a leukocytosis combined with a Glanzmann-type bleeding disorder, resulting from a hematopoietic defect in integrin activation. Symptoms arise from an inability to activate the integrins expressed on hematopoietic cells, including platelets and leukocytes.

  • Sequence similarities

    Belongs to the kindlin family.
    Contains 1 FERM domain.
    Contains 1 PH domain.

  • Domain

    The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.

  • Cellular localization

    Cell projection > podosome. Present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells.
  • Information by UniProt

  • Database links

    • Alternative names

      • Fermitin family homolog 3 antibody
      • Fermitin family member 3 antibody
      • FERMT3 antibody
      • Kind3 antibody
      • Kindlin 3 antibody
      • Kindlin-3 antibody
      • Kindlin3 antibody
      • MGC10966 antibody
      • MIG 2 antibody
      • MIG2 like protein antibody
      • MIG2-like protein antibody
      • MIG2B antibody
      • Unc 112 related protein 2 antibody
      • Unc-112-related protein 2 antibody
      • UNC112C antibody
      • URP2 antibody
      • URP2_HUMAN antibody
      • URP2SF antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)

      IHC image of URP2/Kindlin-3 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab173416, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)
      Western blot - Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)
      All lanes : Anti-URP2/Kindlin-3 antibody [3D6] (ab173416) at 5 µg/ml

      Lane 1 : Human spleen tissue lysate - total protein (ab29699)
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
      Lane 4 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 76 kDa
      Observed band size: 76 kDa
      Additional bands at: 33 kDa (possible cleavage fragment)


      Exposure time: 4 minutes


      We believe that the band observed at approximately 33-kDa is a C-terminal cleavage fragment of URP2/Kindlin-3, which has been described in the literature (PMID 23012377).    Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    References

    This product has been referenced in:

    • Feng C  et al. Expression of kindlin-3 in melanoma cells impedes cell migration and metastasis. Cell Adh Migr 11:419-433 (2017). Read more (PubMed: 27715393) »
    • Qu J  et al. Kindlin-3 interacts with the ribosome and regulates c-Myc expression required for proliferation of chronic myeloid leukemia cells. Sci Rep 5:18491 (2015). IP, ICC/IF ; Human . Read more (PubMed: 26677948) »
    See all 2 Publications for this product

    Publishing research using ab173416? Please let us know so that we can cite the reference in this datasheet.

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