Key features and details
- Rabbit polyclonal to URP2/Kindlin-3
- Suitable for: Flow Cyt, ICC, IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-URP2/Kindlin-3 antibody
See all URP2/Kindlin-3 primary antibodies
DescriptionRabbit polyclonal to URP2/Kindlin-3
Tested applicationsSuitable for: Flow Cyt, ICC, IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- This antibody gave a positive signal in the following Tissue Lysates: Rat Kidney, Rat Thymus, Mouse Spleen This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
Previously labelled as URP2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab68040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).|
FunctionPlays a central role in cell adhesion in hematopoietic cells. Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells. Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs).
Isoform 2 may act as a repressor of NF-kappa-B and apoptosis.
Tissue specificityHighly expressed in lymph node. Expressed in thymus, spleen and leukocytes. Weakly expressed in placenta, small intestine, stomach, testis and lung. Overexpressed in B-cell malignancies.
Involvement in diseaseDefects in FERMT3 are the cause of leukocyte adhesion deficiency type 3 (LAD3) [MIM:612840]; also called leukocyte adhesion deficiency 1 variant (LAD1v). LAD3 is a rare syndrome characterized by infections without pus formation in the presence of a leukocytosis combined with a Glanzmann-type bleeding disorder, resulting from a hematopoietic defect in integrin activation. Symptoms arise from an inability to activate the integrins expressed on hematopoietic cells, including platelets and leukocytes.
Sequence similaritiesBelongs to the kindlin family.
Contains 1 FERM domain.
Contains 1 PH domain.
DomainThe FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
Cellular localizationCell projection > podosome. Present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells.
- Information by UniProt
- Fermitin family homolog 3 antibody
- Fermitin family member 3 antibody
- FERMT3 antibody
All lanes : Anti-URP2/Kindlin-3 antibody (ab68040) at 1 µg/ml
Lane 1 : Kidney (Rat) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lysates/proteins at 10 µg/ml per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 2 minutes
ab68040 used to detect URP2/Kindlin-3 in Immunocytochemisty/Immunofluorescene in mouse platlets spread on fibrinogen. URPE is co-localized to pseudopodial extensions and near membrane edges. Secondy only (anti-rabbit IgG) negative control was also used.
ab68040 staining URP2/Kindlin-3 in Human platelets by Flow Cytometry. Platelets were isolated form Platelet rich plasma and suspended in PBS, fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in 2% BSA for 30 minutes. The sample was incubated with the primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Gating Strategy: Platelets
IHC image of URP2/Kindlin-3 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68040, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab68040 has been referenced in 5 publications.
- Singh D et al. Cooperative Stimulation of Megakaryocytic Differentiation by Gfi1b Gene Targets Kindlin3 and Talin1. PLoS One 11:e0164506 (2016). WB ; Mouse . PubMed: 27768697
- Djaafri I et al. A novel tumor suppressor function of Kindlin-3 in solid cancer. Oncotarget 5:8970-85 (2014). IHC . PubMed: 25344860
- Canault M et al. Human CalDAG-GEFI gene (RASGRP2) mutation affects platelet function and causes severe bleeding. J Exp Med 211:1349-62 (2014). PubMed: 24958846
- Alonso-Orgaz S et al. Proteomic characterization of human coronary thrombus in patients with ST-segment elevation acute myocardial infarction. J Proteomics 109C:368-381 (2014). WB ; Human . PubMed: 25065646
- Dixit N et al. Leukocyte function antigen-1, kindlin-3, and calcium flux orchestrate neutrophil recruitment during inflammation. J Immunol 189:5954-64 (2012). WB ; Human . PubMed: 23144497