Key features and details
- Rabbit polyclonal to Use1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Use1 antibody
See all Use1 primary antibodies
DescriptionRabbit polyclonal to Use1
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human Use1 aa 1-146.
Database link: Q9NZ43
- WB: Mouse heart, liver, brain, stomach and skeletal muscle lysates; Jurkat whole cell lysate. IP: PC-3 whole cell lysate. ICC/IF: U-251 MG cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
- Mouse stomach normal tissue lysate - total protein (ab29256)
- Mouse liver tissue lysate - total protein (ab29301)
- Mouse skeletal muscle tissue lysate - total protein (ab29711)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Mouse brain tissue lysate - total protein (ab4022)
- Jurkat whole cell lysate (ab7899)
Our Abpromise guarantee covers the use of ab234867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 29 kDa.|
|ICC/IF||1/50 - 1/500.|
|IP||1/200 - 1/2000.|
FunctionSNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.
Sequence similaritiesBelongs to the USE1 family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 2010315L10Rik antibody
- 5730403H22Rik antibody
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All lanes : Anti-Use1 antibody (ab234867) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse liver lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse stomach lysate
Lane 6 : Mouse skeletal muscle lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
U-251 MG (human brain glioma cell line) cells stained for Use1 (green) using ab234867 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Use1 was immunoprecipitated from 0.5 mg PC-3 (human prostate adenocarcinoma cell line) whole cell lysate using ab234867 at 1/200 dilution.
Lane 1: Control IgG IP in PC-3 whole cell lysate.
Lane 2: ab234867 IP in PC-3 whole cell lysate.
Lane 3: PC-3 whole cell lysate 10 μg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234867 has not yet been referenced specifically in any publications.