Product nameAnti-Use1 antibody
See all Use1 primary antibodies
DescriptionRabbit polyclonal to Use1
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human Use1 aa 1-146.
Database link: Q9NZ43
- WB: Mouse heart, liver, brain, stomach and skeletal muscle lysates; Jurkat whole cell lysate. IP: PC-3 whole cell lysate. ICC/IF: U-251 MG cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
- Mouse stomach normal tissue lysate - total protein (ab29256)
- Mouse liver tissue lysate - total protein (ab29301)
- Mouse skeletal muscle tissue lysate - total protein (ab29711)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- PC3 whole cell lysate (ab3954)
- Mouse brain tissue lysate - total protein (ab4022)
- Jurkat whole cell lysate (ab7899)
Our Abpromise guarantee covers the use of ab234867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 29 kDa.|
|ICC/IF||1/50 - 1/500.|
|IP||1/200 - 1/2000.|
FunctionSNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.
Sequence similaritiesBelongs to the USE1 family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 2010315L10Rik antibody
- 5730403H22Rik antibody
- AV002165 antibody
All lanes : Anti-Use1 antibody (ab234867) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse liver lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse stomach lysate
Lane 6 : Mouse skeletal muscle lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
U-251 MG (human brain glioma cell line) cells stained for Use1 (green) using ab234867 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Use1 was immunoprecipitated from 0.5 mg PC-3 (human prostate adenocarcinoma cell line) whole cell lysate using ab234867 at 1/200 dilution.
Lane 1: Control IgG IP in PC-3 whole cell lysate.
Lane 2: ab234867 IP in PC-3 whole cell lysate.
Lane 3: PC-3 whole cell lysate 10 μg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
ab234867 has not yet been referenced specifically in any publications.