Overview

  • Product name

  • Description

    Rabbit polyclonal to Use1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Use1 aa 1-146.
    Database link: Q9NZ43

  • Positive control

    • WB: Mouse heart, liver, brain, stomach and skeletal muscle lysates; Jurkat whole cell lysate. IP: PC-3 whole cell lysate. ICC/IF: U-251 MG cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab234867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 29 kDa.
ICC/IF 1/50 - 1/500.
IP 1/200 - 1/2000.

Target

  • Function

    SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.
  • Sequence similarities

    Belongs to the USE1 family.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2010315L10Rik antibody
    • 5730403H22Rik antibody
    • AV002165 antibody
    • D12 antibody
    • Ed2 antibody
    • Embryonic development factor 2 antibody
    • MDS032 antibody
    • P31 antibody
    • Protein D12 antibody
    • Protein p31 antibody
    • Putative MAPK activating protein PM26 antibody
    • Putative MAPK-activating protein PM26 antibody
    • Q-snare antibody
    • RGD1306660 antibody
    • SLT1 antibody
    • SNARE-like tail-anchored protein 1 homolog antibody
    • Unconventional SNARE in the ER 1 homolog (S. cerevisiae) antibody
    • Unconventional SNARE in the ER 1 homolog antibody
    • Use1 antibody
    • USE1 like protein antibody
    • Use1 unconventional SNARE in the ER 1 homolog (S. cerevisiae) antibody
    • USE1-like protein antibody
    • USE1_HUMAN antibody
    • USE1L antibody
    • Vesicle transport protein USE1 antibody
    see all

Images

  • All lanes : Anti-Use1 antibody (ab234867) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Mouse stomach lysate
    Lane 6 : Mouse skeletal muscle lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 29 kDa

  • U-251 MG (human brain glioma cell line) cells stained for Use1 (green) using ab234867 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Use1 was immunoprecipitated from 0.5 mg PC-3 (human prostate adenocarcinoma cell line) whole cell lysate using ab234867 at 1/200 dilution. 

    Lane 1: Control IgG IP in PC-3 whole cell lysate.

    Lane 2: ab234867 IP in PC-3 whole cell lysate.

    Lane 3: PC-3 whole cell lysate 10 μg (Input).

    For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

References

ab234867 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab234867.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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