Product nameAnti-USH1C/Harmonin antibody [EPR8131]
See all USH1C/Harmonin primary antibodies
DescriptionRabbit monoclonal [EPR8131] to USH1C/Harmonin
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human, Spermophilus tridecemlineatus
Synthetic peptide within Human USH1C/Harmonin aa 350-450. The exact sequence is proprietary.
- Human fetal kidney, Human colon and Caco 2 lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.00 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 62 kDa).|
|IP||1/10 - 1/100.|
FunctionMay be involved in protein-protein interaction.
Tissue specificityExpressed in small intestine, colon, kidney, eye and weakly in pancreas. Expressed also in vestibule of the inner ear.
Involvement in diseaseDefects in USH1C are the cause of Usher syndrome type 1C (USH1C) [MIM:276904]; also known as Usher syndrome type I Acadian variety. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness.
Defects in USH1C are the cause of deafness autosomal recessive type 18 (DFNB18) [MIM:602092]. DFNB18 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similaritiesContains 3 PDZ (DHR) domains.
DomainThe PDZ domain 1 mediates interactions with USH1G/SANS and SLC4A7.
- Information by UniProt
- AIE 75 antibody
- AIE75 antibody
- Antigen NY CO 38/NY CO 37 antibody
All lanes : Anti-USH1C/Harmonin antibody [EPR8131] (ab133763) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human colon lysate
Lane 3 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 62 kDa
All lanes : Anti-USH1C/Harmonin antibody [EPR8131] (ab133763) at 1/2500 dilution
Lane 1 : Squirrel cochlea
Lane 2 : Mouse cochlea
Lane 3 : Squirrel colon
Lane 4 : Mouse colon
Lane 5 : Caco2 cells
All lanes : HRP linked Goat anti-rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Additional bands at: 40 kDa (possible isoform), 67 kDa (possible isoform)
Exposure time: 30 seconds
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133763 has not yet been referenced specifically in any publications.