Recombinant
RabMAb

Recombinant Anti-USH1C/Harmonin antibody [EPR8131] (ab133763)

Overview

  • Product name

    Anti-USH1C/Harmonin antibody [EPR8131]
    See all USH1C/Harmonin primary antibodies
  • Description

    Rabbit monoclonal [EPR8131] to USH1C/Harmonin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Spermophilus tridecemlineatus
  • Immunogen

    Synthetic peptide within Human USH1C/Harmonin aa 350-450. The exact sequence is proprietary.

  • Positive control

    • Human fetal kidney, Human colon and Caco 2 lysates.
  • General notes

     

     

     This product was previously labelled as USH1C

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 62 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      May be involved in protein-protein interaction.
    • Tissue specificity

      Expressed in small intestine, colon, kidney, eye and weakly in pancreas. Expressed also in vestibule of the inner ear.
    • Involvement in disease

      Defects in USH1C are the cause of Usher syndrome type 1C (USH1C) [MIM:276904]; also known as Usher syndrome type I Acadian variety. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness.
      Defects in USH1C are the cause of deafness autosomal recessive type 18 (DFNB18) [MIM:602092]. DFNB18 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    • Sequence similarities

      Contains 3 PDZ (DHR) domains.
    • Domain

      The PDZ domain 1 mediates interactions with USH1G/SANS and SLC4A7.
    • Information by UniProt
    • Database links

    • Alternative names

      • AIE 75 antibody
      • AIE75 antibody
      • Antigen NY CO 38/NY CO 37 antibody
      • Antigen NY-CO-38/NY-CO-37 antibody
      • Autoimmune enteropathy related antigen AIE 75 antibody
      • Autoimmune enteropathy related antigen AIE75 antibody
      • Autoimmune enteropathy-related antigen AIE-75 antibody
      • Deafness autosomal recessive 18 antibody
      • DFNB 18 antibody
      • DFNB18 antibody
      • Harmonin antibody
      • NY CO 37 antibody
      • NY CO 38 antibody
      • PDZ 45 antibody
      • PDZ 73 antibody
      • PDZ 73 protein antibody
      • PDZ 73/NY CO 38 antibody
      • PDZ45 antibody
      • PDZ73 antibody
      • PDZ73 protein antibody
      • Protein PDZ-73 antibody
      • Renal carcinoma antigen NY REN 3 antibody
      • Renal carcinoma antigen NY-REN-3 antibody
      • USH 1C antibody
      • USH1C antibody
      • USH1C_HUMAN antibody
      • Ush1cpst antibody
      • Usher syndrome 1C (autosomal recessive severe) antibody
      • Usher syndrome 1C antibody
      • Usher syndrome type 1C protein antibody
      • Usher syndrome type-1C protein antibody
      see all

    Images

    • All lanes : Anti-USH1C/Harmonin antibody [EPR8131] (ab133763) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human colon lysate
      Lane 3 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 62 kDa

    • All lanes : Anti-USH1C/Harmonin antibody [EPR8131] (ab133763) at 1/2500 dilution

      Lane 1 : Squirrel cochlea
      Lane 2 : Mouse cochlea
      Lane 3 : Squirrel colon
      Lane 4 : Mouse colon
      Lane 5 : Caco2 cells

      Secondary
      All lanes : HRP linked Goat anti-rabbit IgG polyclonal at 1/20000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 62 kDa
      Additional bands at: 40 kDa (possible isoform), 67 kDa (possible isoform)


      Exposure time: 30 seconds

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133763 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Colon)
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Glycine)
    Loading amount
    40 µg
    Specification
    Colon
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Mr. James Bjork

    Verified customer

    Submitted Dec 05 2012

    Application
    Western blot
    Sample
    Spermophilus tridecemlineatus Tissue lysate - whole (Cochlea, Colon)
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Glycine)
    Loading amount
    40 µg
    Specification
    Cochlea, Colon
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Mr. James Bjork

    Verified customer

    Submitted Dec 04 2012

    Answer

    Thank you for your enquiry.

    The antibody ab133763 is raised against the sequence AEVHPVPLR KPKYD, the amino acid range 392-405 from human USH1C, which appears to be between the PDZ2 and PDZ3 domains, according to the UniProt entry at this link: http://www.uniprot.org/uniprot/Q9Y6N9 .

    The antibody failed validation in immunocytochemistry and and immunohistochemistry on parrafin-embedded tissue sections. The reactivity with mouse protein was determined by western blotting. I have not seen the data but if you are still interested, I will be happy to have the laboratory forward it.

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