Recombinant Anti-USH1C/Harmonin antibody [EPR8131] (ab133763)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8131] to USH1C/Harmonin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-USH1C/Harmonin antibody [EPR8131]
See all USH1C/Harmonin primary antibodies -
Description
Rabbit monoclonal [EPR8131] to USH1C/Harmonin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Spermophilus tridecemlineatus -
Immunogen
Synthetic peptide within Human USH1C/Harmonin aa 350-450. The exact sequence is proprietary.
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Positive control
- Human fetal kidney, Human colon and Caco 2 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8131 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 62 kDa).
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 62 kDa). |
Target
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Function
May be involved in protein-protein interaction. -
Tissue specificity
Expressed in small intestine, colon, kidney, eye and weakly in pancreas. Expressed also in vestibule of the inner ear. -
Involvement in disease
Defects in USH1C are the cause of Usher syndrome type 1C (USH1C) [MIM:276904]; also known as Usher syndrome type I Acadian variety. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness.
Defects in USH1C are the cause of deafness autosomal recessive type 18 (DFNB18) [MIM:602092]. DFNB18 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
Sequence similarities
Contains 3 PDZ (DHR) domains. -
Domain
The PDZ domain 1 mediates interactions with USH1G/SANS and SLC4A7. - Information by UniProt
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Database links
- Entrez Gene: 10083 Human
- Entrez Gene: 72088 Mouse
- Entrez Gene: 308596 Rat
- Omim: 605242 Human
- SwissProt: Q9Y6N9 Human
- SwissProt: Q9ES64 Mouse
- SwissProt: Q3T1I3 Rat
- Unigene: 502072 Human
see all -
Alternative names
- AIE 75 antibody
- AIE75 antibody
- Antigen NY CO 38/NY CO 37 antibody
see all
Images
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All lanes : Anti-USH1C/Harmonin antibody [EPR8131] (ab133763) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human colon lysate
Lane 3 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 62 kDa -
All lanes : Anti-USH1C/Harmonin antibody [EPR8131] (ab133763) at 1/2500 dilution
Lane 1 : Squirrel cochlea
Lane 2 : Mouse cochlea
Lane 3 : Squirrel colon
Lane 4 : Mouse colon
Lane 5 : Caco2 cells
Secondary
All lanes : HRP linked Goat anti-rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Additional bands at: 40 kDa (possible isoform), 67 kDa (possible isoform)
Exposure time: 30 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133763 has been referenced in 1 publication.
- Al-Choboq J et al. Usher Syndrome Belongs to the Genetic Diseases Associated with Radiosensitivity: Influence of the ATM Protein Kinase. Int J Mol Sci 23:N/A (2022). PubMed: 35163494