Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8131] to USH1C/Harmonin - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-USH1C/Harmonin antibody [EPR8131] - BSA and Azide free
See all USH1C/Harmonin primary antibodies
DescriptionRabbit monoclonal [EPR8131] to USH1C/Harmonin - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Spermophilus tridecemlineatus
Synthetic peptide within Human USH1C/Harmonin aa 350-450. The exact sequence is proprietary.
ab248645 is the carrier-free version of ab133763 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248645 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as USH1C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Dissociation constant (KD)KD = 4.00 x 10 -11 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab248645 in the following tested applications.
|WB||Use at an assay dependent concentration. Detects a band of approximately 73 kDa (predicted molecular weight: 62 kDa).|
FunctionMay be involved in protein-protein interaction.
Tissue specificityExpressed in small intestine, colon, kidney, eye and weakly in pancreas. Expressed also in vestibule of the inner ear.
Involvement in diseaseDefects in USH1C are the cause of Usher syndrome type 1C (USH1C) [MIM:276904]; also known as Usher syndrome type I Acadian variety. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness.
Defects in USH1C are the cause of deafness autosomal recessive type 18 (DFNB18) [MIM:602092]. DFNB18 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similaritiesContains 3 PDZ (DHR) domains.
DomainThe PDZ domain 1 mediates interactions with USH1G/SANS and SLC4A7.
- Information by UniProt
- AIE 75 antibody
- AIE75 antibody
- Antigen NY CO 38/NY CO 37 antibody
All lanes : Anti-USH1C/Harmonin antibody [EPR8131] (ab133763) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human colon lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 62 kDa
This data was developed using ab133763, the same antibody clone in a different buffer formulation.
ab248645 has not yet been referenced specifically in any publications.