Key features and details
- Rabbit polyclonal to USH1G
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-USH1G antibody
See all USH1G primary antibodies
DescriptionRabbit polyclonal to USH1G
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human thyroid gland tissue; RT-4 and U-251 MG cell lysates, Human liver and tonsil tissue lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500. Predicted molecular weight: 51 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
FunctionRequired for normal development and maintenance of cochlear hair cell bundles. Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing.
Tissue specificityExpressed in vestibule of the inner ear, eye and small intestine.
Involvement in diseaseUsher syndrome 1G
Sequence similaritiesContains 3 ANK repeats.
Contains 1 SAM (sterile alpha motif) domain.
Cellular localizationCytoplasm > cytosol. Cytoplasm > cytoskeleton. Cell membrane. Detected at the tip of cochlear hair cell stereocilia. Recruited to the cell membrane via interaction with CDH23 or PCDH15.
- Information by UniProt
- ANKS 4A antibody
- ANKS4A antibody
- FLJ33924 antibody
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles. Recommended concentration of ab150820 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue tissue, labelling USH1G with ab150820 at 1/1000 dilution.
All lanes : Anti-USH1G antibody (ab150820) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 51 kDa
ab150820 has not yet been referenced specifically in any publications.