Overview

  • Product name

  • Description

    Rabbit polyclonal to USH1G
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to internal sequence amino acids 90-163 of Human USH1G (UniProt Q495M9).

  • Positive control

    • Human thyroid gland tissue; RT-4 and U-251 MG cell lysates, Human liver and tonsil tissue lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500. Predicted molecular weight: 51 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function

    Required for normal development and maintenance of cochlear hair cell bundles. Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing.
  • Tissue specificity

    Expressed in vestibule of the inner ear, eye and small intestine.
  • Involvement in disease

    Usher syndrome 1G
  • Sequence similarities

    Contains 3 ANK repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization

    Cytoplasm > cytosol. Cytoplasm > cytoskeleton. Cell membrane. Detected at the tip of cochlear hair cell stereocilia. Recruited to the cell membrane via interaction with CDH23 or PCDH15.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANKS 4A antibody
    • ANKS4A antibody
    • FLJ33924 antibody
    • SANS antibody
    • Scaffold protein containing ankyrin repeats and SAM domain antibody
    • USH 1G antibody
    • USH1G antibody
    • USH1G_HUMAN antibody
    • Usher syndrome 1G (autosomal recessive) antibody
    • Usher syndrome type 1G protein antibody
    • Usher syndrome type-1G protein antibody
    see all

Images

  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles. Recommended concentration of ab150820 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue tissue, labelling USH1G with ab150820 at 1/1000 dilution.
  • All lanes : Anti-USH1G antibody (ab150820) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Predicted band size: 51 kDa

References

ab150820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab150820.
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