Overview

  • Product name

  • Description

    Rabbit polyclonal to USO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human USO1 aa 36-177.
    Database link: AAH3265

  • Positive control

    • A431, 293T, Jurkat and Raji cell lysates; Lung Adenocarcinoma tissue
  • General notes

    Previously labelled as Vesicle docking protein p115. 

Properties

Applications

Our Abpromise guarantee covers the use of ab102470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/5000 - 1/20000. Predicted molecular weight: 108 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0).

Target

  • Function

    General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
  • Sequence similarities

    Belongs to the VDP/USO1/EDE1 family.
    Contains 10 ARM repeats.
  • Domain

    Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
  • Post-translational
    modifications

    Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
  • Cellular localization

    Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • General vesicular transport factor antibody
    • General vesicular transport factor p115 antibody
    • P115 antibody
    • Protein USO1 homolog antibody
    • TAP antibody
    • Transcytosis associated protein antibody
    • Transcytosis-associated protein antibody
    • Uso1 antibody
    • USO1_HUMAN antibody
    • VDP antibody
    • Vesicle docking protein antibody
    • Vesicle docking protein p115 antibody
    • Vesicle-docking protein antibody
    see all

Images

  • Anti-USO1 antibody (ab102470) at 1/10000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 108 kDa



    7.5% SDS-PAGE.
  • ab102470 at 1/500 dilution staining USO1 in Human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

  • ab102470 staining USO1 in human HeLa cells using ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% donkey serum in 0.1% PBST for 1 hour at 22oC. Samples were incubated with primary antibody (1/50 in 1% donkey serum in 0.1% PBST) for 3 hours at 22oC. Alexa Fluor® 594 conjugated Donkey-anti-Rb polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Howley BV  et al. A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer. Oncogene 37:1308-1325 (2018). Read more (PubMed: 29249802) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mice Hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
35 µg
Specification
Mice Hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Feb 18 2019

Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
20 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 20 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
HeLa cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 19 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-15% gradient SDS-PAGE)
Loading amount
15 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Nov 14 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Panc-1)
Permeabilization
No
Specification
Panc-1
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Apr 28 2011

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