Overview

  • Product name

  • Description

    Rabbit polyclonal to USO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human USO1 aa 717-962. The exact sequence is proprietary.
    Database link: O60763

  • Positive control

    • 293T , A431 , HeLa , HepG2 whole cell lysates, A431 cells
  • General notes

    Previously labelled as Vesicle docking protein p115. 

Properties

Applications

Our Abpromise guarantee covers the use of ab184014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 108 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
  • Sequence similarities

    Belongs to the VDP/USO1/EDE1 family.
    Contains 10 ARM repeats.
  • Domain

    Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
  • Post-translational
    modifications

    Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
  • Cellular localization

    Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • General vesicular transport factor antibody
    • General vesicular transport factor p115 antibody
    • P115 antibody
    • Protein USO1 homolog antibody
    • TAP antibody
    • Transcytosis associated protein antibody
    • Transcytosis-associated protein antibody
    • Uso1 antibody
    • USO1_HUMAN antibody
    • VDP antibody
    • Vesicle docking protein antibody
    • Vesicle docking protein p115 antibody
    • Vesicle-docking protein antibody
    see all

Images

  • All lanes : Anti-USO1 antibody (ab184014) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 108 kDa



    7.5% SDS PAGE

  • Immunocytochemical analysis of methanol fixed A431 cells labeling USO1 at 1/500 dilution (green) and counterstained with Hoechst 33342. Cells were fixed in 100% methanol for 5 mins.

References

This product has been referenced in:

  • Zhong B  et al. Hsp20 Protects against Oxygen-Glucose Deprivation/Reperfusion-Induced Golgi Fragmentation and Apoptosis through Fas/FasL Pathway. Oxid Med Cell Longev 2015:606934 (2015). WB . Read more (PubMed: 26199678) »
See 1 Publication for this product

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