• Product name

  • Description

    Rabbit polyclonal to USO1
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human USO1 aa 717-962. The exact sequence is proprietary.
    Database link: O60763

  • Positive control

    • 293T , A431 , HeLa , HepG2 whole cell lysates, A431 cells
  • General notes

    Previously labelled as Vesicle docking protein p115. 



Our Abpromise guarantee covers the use of ab184014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 108 kDa.
ICC/IF 1/100 - 1/1000.


  • Function

    General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
  • Sequence similarities

    Belongs to the VDP/USO1/EDE1 family.
    Contains 10 ARM repeats.
  • Domain

    Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
  • Post-translational

    Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
  • Cellular localization

    Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • General vesicular transport factor antibody
    • General vesicular transport factor p115 antibody
    • P115 antibody
    • Protein USO1 homolog antibody
    • TAP antibody
    • Transcytosis associated protein antibody
    • Transcytosis-associated protein antibody
    • Uso1 antibody
    • USO1_HUMAN antibody
    • VDP antibody
    • Vesicle docking protein antibody
    • Vesicle docking protein p115 antibody
    • Vesicle-docking protein antibody
    see all


  • All lanes : Anti-USO1 antibody (ab184014) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 108 kDa

    7.5% SDS PAGE

  • Immunocytochemical analysis of methanol fixed A431 cells labeling USO1 at 1/500 dilution (green) and counterstained with Hoechst 33342. Cells were fixed in 100% methanol for 5 mins.


This product has been referenced in:

  • Zhong B  et al. Hsp20 Protects against Oxygen-Glucose Deprivation/Reperfusion-Induced Golgi Fragmentation and Apoptosis through Fas/FasL Pathway. Oxid Med Cell Longev 2015:606934 (2015). WB . Read more (PubMed: 26199678) »
See 1 Publication for this product

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