Key features and details
- Rabbit polyclonal to USO1 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-USO1 antibody - C-terminal
See all USO1 primary antibodies
DescriptionRabbit polyclonal to USO1 - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human USO1 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: O60763
- IHC-P: SCC15 xenograft tissue. WB: HeLa, HepG2 and MOLT-4 whole cell lysates.
Previously labelled as Vesicle docking protein p115.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 108 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionGeneral vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Sequence similaritiesBelongs to the VDP/USO1/EDE1 family.
Contains 10 ARM repeats.
DomainComposed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
modificationsPhosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
Cellular localizationCytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
- Information by UniProt
- General vesicular transport factor antibody
- General vesicular transport factor p115 antibody
- P115 antibody
All lanes : Anti-USO1 antibody - C-terminal (ab228648) at 1/10000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 108 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded SCC15 xenograft tissue stained for USO1 using ab228648 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228648 has not yet been referenced specifically in any publications.