Overview

  • Product name

    Anti-USO1 antibody - C-terminal
    See all USO1 primary antibodies
  • Description

    Rabbit polyclonal to USO1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human USO1 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: O60763

  • Positive control

    • IHC-P: SCC15 xenograft tissue. WB: HeLa, HepG2 and MOLT-4 whole cell lysates.
  • General notes

    Previously labelled as Vesicle docking protein p115. 

Properties

Applications

Our Abpromise guarantee covers the use of ab228648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 108 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
  • Sequence similarities

    Belongs to the VDP/USO1/EDE1 family.
    Contains 10 ARM repeats.
  • Domain

    Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
  • Post-translational
    modifications

    Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
  • Cellular localization

    Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • General vesicular transport factor antibody
    • General vesicular transport factor p115 antibody
    • P115 antibody
    • Protein USO1 homolog antibody
    • TAP antibody
    • Transcytosis associated protein antibody
    • Transcytosis-associated protein antibody
    • Uso1 antibody
    • USO1_HUMAN antibody
    • VDP antibody
    • Vesicle docking protein antibody
    • Vesicle docking protein p115 antibody
    • Vesicle-docking protein antibody
    see all

Images

  • All lanes : Anti-USO1 antibody - C-terminal (ab228648) at 1/10000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 108 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded SCC15 xenograft tissue stained for USO1 using ab228648 at 1/100 dilution in immunohistochemical analysis.

References

ab228648 has not yet been referenced specifically in any publications.

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