Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2140Y] to USO1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-USO1 antibody [EPR2140Y]
See all USO1 primary antibodies
DescriptionRabbit monoclonal [EPR2140Y] to USO1
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human USO1 aa 1-100 (N terminal). The exact sequence is proprietary.
- WB: HeLa cell lysate; 293T cell lysate; HepG2 cell lysate; Cos cell lysate
Previously labelled as Vesicle docking protein p115.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab68137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).|
FunctionGeneral vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Sequence similaritiesBelongs to the VDP/USO1/EDE1 family.
Contains 10 ARM repeats.
DomainComposed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
modificationsPhosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
Cellular localizationCytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
- Information by UniProt
- General vesicular transport factor antibody
- General vesicular transport factor p115 antibody
- P115 antibody
All lanes : Anti-USO1 antibody [EPR2140Y] (ab68137) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Cos cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
ab68137 has been referenced in 1 publication.
- Jin Y & Dai Z USO1 promotes tumor progression via activating Erk pathway in multiple myeloma cells. Biomed Pharmacother 78:264-71 (2016). WB ; Human . PubMed: 26898451