Key features and details
- Rabbit polyclonal to USP1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-USP1 antibody
See all USP1 primary antibodies
DescriptionRabbit polyclonal to USP1
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 450 and 500 of human USP1 (NP_003359.3)
- Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Prostate Carcinoma, Small Cell Lung Cancer
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab84772 was affinity purified using an epitope specific to USP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab84772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Epitope exposure is recommended.
Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionNegative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
Sequence similaritiesBelongs to the peptidase C19 family.
Developmental stageCell cycle-regulated. Highest level during S phase.
modificationsAutocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
Ubiquitinated; leading to its subsequent proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Deubiquitinating enzyme 1 antibody
- hUBP antibody
- Ubiquitin carboxyl terminal hydrolase 1 antibody
ab84772 has not yet been referenced specifically in any publications.