Anti-USP1 antibody (ab84772)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP1 antibody (ab84772)

    Key features and details

    • Rabbit polyclonal to USP1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-USP1 antibody
      See all USP1 primary antibodies

    • Description

      Rabbit polyclonal to USP1

    • Host species

      Rabbit

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

    • Immunogen

      Synthetic peptide corresponding to a region between residue 450 and 500 of human USP1 (NP_003359.3)

    • Positive control

      • Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Prostate Carcinoma, Small Cell Lung Cancer

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab84772 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.

    • Sequence similarities

      Belongs to the peptidase C19 family.

    • Developmental stage

      Cell cycle-regulated. Highest level during S phase.

    • Post-translational
      modifications

      Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
      Ubiquitinated; leading to its subsequent proteasomal degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Deubiquitinating enzyme 1 antibody
      • hUBP antibody
      • Ubiquitin carboxyl terminal hydrolase 1 antibody
      • Ubiquitin carboxyl-terminal hydrolase 1 antibody
      • Ubiquitin specific peptidase 1 antibody
      • Ubiquitin specific protease 1 antibody
      • Ubiquitin thioesterase 1 antibody
      • Ubiquitin thiolesterase 1 antibody
      • Ubiquitin-specific-processing protease 1 antibody
      • UBP antibody
      • UBP1_HUMAN antibody
      • USP 1 antibody
      • Usp1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP1 antibody (ab84772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP1 antibody (ab84772)
      Staining of USP1 by Immunohistochemistry on a formalin fixed, paraffin embedded section of human thyroid carcinoma using a 1/250 dilution of ab84772. Detection: DAB staining

    References (0)

    Publishing research using ab84772? Please let us know so that we can cite the reference in this datasheet.

    ab84772 has not yet been referenced specifically in any publications.

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