Anti-USP1 antibody (ab84772)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-USP1 antibody
See all USP1 primary antibodies -
Description
Rabbit polyclonal to USP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between residue 450 and 500 of human USP1 (NP_003359.3)
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Positive control
- Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Prostate Carcinoma, Small Cell Lung Cancer
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab84772 was affinity purified using an epitope specific to USP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab84772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Application notes
IHC-P: 1/100 - 1/500.
Epitope exposure is recommended.
Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Epitope exposure is recommended.
Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. -
Sequence similarities
Belongs to the peptidase C19 family. -
Developmental stage
Cell cycle-regulated. Highest level during S phase. -
Post-translational
modificationsAutocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
Ubiquitinated; leading to its subsequent proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 516328 Cow
- Entrez Gene: 7398 Human
- Entrez Gene: 230484 Mouse
- Entrez Gene: 313387 Rat
- Omim: 603478 Human
- SwissProt: Q29RP1 Cow
- SwissProt: O94782 Human
- SwissProt: Q8BJQ2 Mouse
see all -
Alternative names
- Deubiquitinating enzyme 1 antibody
- hUBP antibody
- Ubiquitin carboxyl terminal hydrolase 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP1 antibody (ab84772)Staining of USP1 by Immunohistochemistry on a formalin fixed, paraffin embedded section of human thyroid carcinoma using a 1/250 dilution of ab84772. Detection: DAB staining
Protocols
Datasheets and documents
References
ab84772 has not yet been referenced specifically in any publications.
