Product nameAnti-USP1 antibody - C-terminal
See all USP1 primary antibodies
DescriptionRabbit polyclonal to USP1 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide within Human USP1 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: O94782
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 88 kDa.|
FunctionNegative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
Sequence similaritiesBelongs to the peptidase C19 family.
Developmental stageCell cycle-regulated. Highest level during S phase.
modificationsAutocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
Ubiquitinated; leading to its subsequent proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Deubiquitinating enzyme 1 antibody
- hUBP antibody
- Ubiquitin carboxyl terminal hydrolase 1 antibody
All lanes : Anti-USP1 antibody - C-terminal (ab227551) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa
5% SDS-PAGE gel.
ab227551 has not yet been referenced specifically in any publications.