Product nameAnti-USP10 antibody
See all USP10 primary antibodies
DescriptionRabbit polyclonal to USP10
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Human
Recombinant GST fusion protein (mouse Usp10). This antibody was generated against amino acids 1-300 of mouse USP10.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab72486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution (PMID 20096447).
WB: 1/1000. Predicted molecular weight: 87 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the peptidase C19 family. USP10 subfamily.
modificationsPhosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
Cellular localizationCytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.
- Information by UniProt
- Deubiquitinating enzyme 10 antibody
- KIAA0190 antibody
- MGC124997 antibody
All lanes : Anti-USP10 antibody (ab72486) at 1/1000 dilution
Lane 1 : HEK293
Lane 2 : HEK293/USP10
Predicted band size: 87 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Song JS et al. Dual loss of USP10 and p14ARF protein expression is associated with poor prognosis in patients with small intestinal adenocarcinoma. Tumour Biol 40:1010428318808678 (2018). Read more (PubMed: 30375264) »
- Zhang M et al. Ubiquitin-specific Peptidase 10 (USP10) Deubiquitinates and Stabilizes MutS Homolog 2 (MSH2) to Regulate Cellular Sensitivity to DNA Damage. J Biol Chem 291:10783-91 (2016). Read more (PubMed: 26975374) »