Anti-USP10 antibody (ab72486)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-USP10 antibody
See all USP10 primary antibodies -
Description
Rabbit polyclonal to USP10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant GST fusion protein (mouse Usp10). This antibody was generated against amino acids 1-300 of mouse USP10.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab72486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | ||
| WB | ||
| ICC/IF | ||
| IP |
Application notes
ICC/IF: 1/200.
IHC-P: Use at an assay dependent dilution (PMID 20096447).
IP: 1/500.
WB: 1/1000. Predicted molecular weight: 87 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution (PMID 20096447).
IP: 1/500.
WB: 1/1000. Predicted molecular weight: 87 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the peptidase C19 family. USP10 subfamily. -
Post-translational
modificationsPhosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus. -
Cellular localization
Cytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM. - Information by UniProt
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Database links
- Entrez Gene: 9100 Human
- Entrez Gene: 22224 Mouse
- Omim: 609818 Human
- SwissProt: Q14694 Human
- SwissProt: P52479 Mouse
- Unigene: 136778 Human
- Unigene: 256910 Mouse
- Unigene: 421337 Mouse
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Alternative names
- Deubiquitinating enzyme 10 antibody
- KIAA0190 antibody
- MGC124997 antibody
see all
Images
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Western blot - Anti-USP10 antibody (ab72486)All lanes : Anti-USP10 antibody (ab72486) at 1/1000 dilution
Lane 1 : HEK293
Lane 2 : HEK293/USP10
Predicted band size: 87 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Song JS et al. Dual loss of USP10 and p14ARF protein expression is associated with poor prognosis in patients with small intestinal adenocarcinoma. Tumour Biol 40:1010428318808678 (2018). Read more (PubMed: 30375264) »
- Zhang M et al. Ubiquitin-specific Peptidase 10 (USP10) Deubiquitinates and Stabilizes MutS Homolog 2 (MSH2) to Regulate Cellular Sensitivity to DNA Damage. J Biol Chem 291:10783-91 (2016). Read more (PubMed: 26975374) »
