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    usp10-antibody-ab72486.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Deubiquitination
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Anti-USP10 antibody (ab72486)

  • Datasheet
Reviews (1) Submit a question References (9)

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Western blot - Anti-USP10 antibody (ab72486)

    Key features and details

    • Rabbit polyclonal to USP10
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Recombinant human USP10 protein (ab198669)
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    Anti-Nucleolin antibody (ab22758)

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    Overview

    • Product name

      Anti-USP10 antibody
      See all USP10 primary antibodies
    • Description

      Rabbit polyclonal to USP10
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant GST fusion protein (mouse Usp10). This antibody was generated against amino acids 1-300 of mouse USP10.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: Whole serum
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Deubiquitination
      • Epigenetics and Nuclear Signaling
      • Ubiquitin & Ubiquitin Like Modifiers
      • Deubiquitination

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human USP10 protein (ab198669)

    Applications

    Our Abpromise guarantee covers the use of ab72486 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
  • Application notes
    ICC/IF: 1/200.
    IHC-P: Use at an assay dependent dilution (PMID 20096447).
    IP: 1/500.
    WB: 1/1000. Predicted molecular weight: 87 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the peptidase C19 family. USP10 subfamily.
    • Post-translational
      modifications

      Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
    • Cellular localization

      Cytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.
    • Target information above from: UniProt accession Q14694 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9100 Human
      • Omim: 609818 Human
      • SwissProt: Q14694 Human
      • Unigene: 136778 Human
      • Alternative names

        • Deubiquitinating enzyme 10 antibody
        • KIAA0190 antibody
        • MGC124997 antibody
        • MGC2621 antibody
        • Ubiquitin carboxyl terminal hydrolase 10 antibody
        • Ubiquitin carboxyl-terminal hydrolase 10 antibody
        • Ubiquitin specific peptidase 10 antibody
        • Ubiquitin specific processing protease 10 antibody
        • Ubiquitin specific protease 10 antibody
        • Ubiquitin thioesterase 10 antibody
        • Ubiquitin thiolesterase 10 antibody
        • Ubiquitin-specific-processing protease 10 antibody
        • UBP10_HUMAN antibody
        • UBPO antibody
        • USP 10 antibody
        • USP10 antibody
        see all

      Images

      • Western blot - Anti-USP10 antibody (ab72486)
        Western blot - Anti-USP10 antibody (ab72486)
        All lanes : Anti-USP10 antibody (ab72486) at 1/1000 dilution

        Lane 1 : HEK293
        Lane 2 : HEK293/USP10

        Predicted band size: 87 kDa
        Observed band size: 100 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (9)

      Publishing research using ab72486? Please let us know so that we can cite the reference in this datasheet.

      ab72486 has been referenced in 9 publications.

      • Kim K  et al. Prognostic significance of USP10 and p14ARF expression in patients with colorectal cancer. Pathol Res Pract 216:152988 (2020). PubMed: 32362421
      • Xie W  et al. Auto-ubiquitination of NEDD4-1 Recruits USP13 to Facilitate Autophagy through Deubiquitinating VPS34. Cell Rep 30:2807-2819.e4 (2020). PubMed: 32101753
      • Hu C  et al. The USP10-HDAC6 axis confers cisplatin resistance in non-small cell lung cancer lacking wild-type p53. Cell Death Dis 11:328 (2020). PubMed: 32382008
      • Song JS  et al. Dual loss of USP10 and p14ARF protein expression is associated with poor prognosis in patients with small intestinal adenocarcinoma. Tumour Biol 40:1010428318808678 (2018). PubMed: 30375264
      • Zhang M  et al. Ubiquitin-specific Peptidase 10 (USP10) Deubiquitinates and Stabilizes MutS Homolog 2 (MSH2) to Regulate Cellular Sensitivity to DNA Damage. J Biol Chem 291:10783-91 (2016). PubMed: 26975374
      • Li L  et al. A specific E3 ligase/deubiquitinase pair modulates TBP protein levels during muscle differentiation. Elife 4:e08536 (2015). PubMed: 26393420
      • Xia HG  et al. Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death. J Cell Biol 210:705-16 (2015). PubMed: 26323688
      • Yuan J  et al. USP10 regulates p53 localization and stability by deubiquitinating p53. Cell 140:384-96 (2010). IHC-P ; Human . PubMed: 20096447
      • Boulkroun S  et al. Vasopressin-inducible ubiquitin-specific protease 10 increases ENaC cell surface expression by deubiquitylating and stabilizing sorting nexin 3. Am J Physiol Renal Physiol 295:F889-900 (2008). PubMed: 18632802

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunocytochemistry/ Immunofluorescence abreview for Anti-USP10 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Methanol
      Permeabilization
      No
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Feb 01 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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