Overview

  • Product name

    Anti-USP10 antibody [EPR4261] - BSA and Azide free
    See all USP10 primary antibodies
  • Description

    Rabbit monoclonal [EPR4261] to USP10 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human USP10 aa 1-100. The exact sequence is proprietary.

  • General notes

    ab239953 is the carrier-free version of ab109219 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239953 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Antigen retrieval is recommended.

 

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 87 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the peptidase C19 family. USP10 subfamily.
    • Post-translational
      modifications

      Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
    • Cellular localization

      Cytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.
    • Information by UniProt
    • Database links

    • Alternative names

      • Deubiquitinating enzyme 10 antibody
      • KIAA0190 antibody
      • MGC124997 antibody
      • MGC2621 antibody
      • Ubiquitin carboxyl terminal hydrolase 10 antibody
      • Ubiquitin carboxyl-terminal hydrolase 10 antibody
      • Ubiquitin specific peptidase 10 antibody
      • Ubiquitin specific processing protease 10 antibody
      • Ubiquitin specific protease 10 antibody
      • Ubiquitin thioesterase 10 antibody
      • Ubiquitin thiolesterase 10 antibody
      • Ubiquitin-specific-processing protease 10 antibody
      • UBP10_HUMAN antibody
      • UBPO antibody
      • USP 10 antibody
      • USP10 antibody
      see all

    Images

    • ICC/IF image of ab109219 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109219 at 1/50 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109219).

    • ab109219, at a 1/250 dilution, staining USP10 in paraffin embedded Human colon tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109219).

    References

    ab239953 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab239953.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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