Product nameAnti-USP13 antibody
See all USP13 primary antibodies
DescriptionRabbit polyclonal to USP13
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human USP13 (NP_003931.2).
- HeLa and 293T whole cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 97 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
Tissue specificityHighly expressed in ovary and testes.
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 2 UBA domains.
Contains 1 UBP-type zinc finger.
- Information by UniProt
- Deubiquitinating enzyme 13 antibody
- Isopeptidase T 3 antibody
- Isopeptidase T-3 antibody
All lanes : Anti-USP13 antibody (ab99421) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma tissues labelling USP13 with ab99421 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
ICC/IF image of ab99421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99421 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab99421 at 1 µg/ml detecting USP13 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 10 second exposure.
For immunoprecipitation, ab99421 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1; IP using ab99421
Lane 2; IP using ab99423, a rabbit anti-USP13 antibody which recognizes a downstream epitope.
Lane 3; IP using control IgG.
This product has been referenced in:
- Pesce E et al. The Autophagy Inhibitor Spautin-1 Antagonizes Rescue of Mutant CFTR Through an Autophagy-Independent and USP13-Mediated Mechanism. Front Pharmacol 9:1464 (2018). Read more (PubMed: 30618756) »
- Geng J et al. Down-regulation of USP13 mediates phenotype transformation of fibroblasts in idiopathic pulmonary fibrosis. Respir Res 16:124 (2015). WB, IP . Read more (PubMed: 26453058) »