Recombinant Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)
![Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)](https://www.abcam.com/ps/products/247/ab247812/Images/ab247812-410638-anti-usp13-antibody-epr4348-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4348] to USP13 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-USP13 antibody [EPR4348] - BSA and Azide free
See all USP13 primary antibodies -
Description
Rabbit monoclonal [EPR4348] to USP13 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247812 is the carrier-free version of ab109264.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4348 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
|
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| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Tissue specificity
Highly expressed in ovary and testes. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 2 UBA domains.
Contains 1 UBP-type zinc finger. - Information by UniProt
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Database links
- Entrez Gene: 8975 Human
- Entrez Gene: 72607 Mouse
- Omim: 603591 Human
- SwissProt: Q92995 Human
- SwissProt: Q5BKP2 Mouse
- Unigene: 175322 Human
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Alternative names
- Deubiquitinating enzyme 13 antibody
- Isopeptidase T 3 antibody
- Isopeptidase T-3 antibody
see all
Images
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Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)
This data was developed using ab109264, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: USP13 knockout HAP1 cell lysate (20 µg)
Lane 3: A375 cell lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109264 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109264 was shown to recognize USP13 when USP13 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP13 knockout samples were subjected to SDS-PAGE. ab109264 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)](https://www.abcam.com/ps/products/247/ab247812/Images/ab247812-3-anti-usp13-antibody-epr4348-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)This data was developed using ab109264, the same antibody clone in a different buffer formulation.Overlay histogram showing SHSY-5Y cells stained with ab109264 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109264, 1/9400 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)](https://www.abcam.com/ps/products/247/ab247812/Images/ab247812-398407-anti-usp13-antibody-epr4348-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)All lanes : Anti-USP13 antibody [EPR4348] (ab109264) at 1/1000 dilution
Lane 1 : SH SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 97 kDa
Observed band size: 97 kDaThis data was developed using ab109264, the same antibody clone in a different buffer formulation.
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![Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)](https://www.abcam.com/ps/products/247/ab247812/Images/ab247812-6-benefits-of-recombinant-antibodies.png)
Anti-USP13 antibody [EPR4348] - BSA and Azide free (ab247812)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247812 has not yet been referenced specifically in any publications.
