Overview

  • Product name

  • Description

    Rabbit polyclonal to USP14/TGT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human USP14/TGT aa 286-494.

  • Positive control

    • Human breast carcinoma tissue; A431, Jurkat and Mouse brain whole cell lysates; Rat brain lysate.
  • General notes

     This product was previously labelled as USP14

     

Properties

Applications

Our Abpromise guarantee covers the use of ab137432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).
  • Sequence similarities

    Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
    Contains 1 ubiquitin-like domain.
  • Cellular localization

    Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 14 antibody
    • TGT antibody
    • tRNA guanine transglycosylase 60 kD subunit antibody
    • tRNA guanine transglycosylase antibody
    • Ubiquitin carboxyl terminal hydrolase 14 antibody
    • Ubiquitin carboxyl-terminal hydrolase 14 antibody
    • Ubiquitin specific peptidase 14 antibody
    • Ubiquitin specific processing protease 14 antibody
    • Ubiquitin specific protease 14 antibody
    • Ubiquitin thiolesterase 14 antibody
    • Ubiquitin-specific-processing protease 14 antibody
    • UBP14_HUMAN antibody
    • USP 14 antibody
    • USP14 antibody
    see all

Images

  • All lanes : Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : USP14/TGT Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 56 kDa

  • Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 56 kDa



    7.5% SDS PAGE

  • USP14/TGT was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab137432.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 56kDa; USP14/TGT

  • All lanes : Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Jurkat whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 56 kDa



    7.5% SDS PAGE
  • Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution + Rat brain cell lyaste at 50 µg

    Predicted band size: 56 kDa



    7.5 % SDS-PAGE
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling USP14/TGT with ab137432 at 1/500 dilution.

References

ab137432 has not yet been referenced specifically in any publications.

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