• Product name

  • Description

    Rabbit polyclonal to USP14/TGT
  • Host species

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human USP14/TGT aa 286-494.

  • Positive control

    • Human breast carcinoma tissue; A431, Jurkat and Mouse brain whole cell lysates; Rat brain lysate.
  • General notes

     This product was previously labelled as USP14




Our Abpromise guarantee covers the use of ab137432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).


  • Function

    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).
  • Sequence similarities

    Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
    Contains 1 ubiquitin-like domain.
  • Cellular localization

    Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 14 antibody
    • TGT antibody
    • tRNA guanine transglycosylase 60 kD subunit antibody
    • tRNA guanine transglycosylase antibody
    • Ubiquitin carboxyl terminal hydrolase 14 antibody
    • Ubiquitin carboxyl-terminal hydrolase 14 antibody
    • Ubiquitin specific peptidase 14 antibody
    • Ubiquitin specific processing protease 14 antibody
    • Ubiquitin specific protease 14 antibody
    • Ubiquitin thiolesterase 14 antibody
    • Ubiquitin-specific-processing protease 14 antibody
    • UBP14_HUMAN antibody
    • USP 14 antibody
    • USP14 antibody
    see all


  • All lanes : Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : USP14/TGT Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 56 kDa

  • Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 56 kDa

    7.5% SDS PAGE

  • USP14/TGT was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab137432.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 56kDa; USP14/TGT

  • All lanes : Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Jurkat whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 56 kDa

    7.5% SDS PAGE
  • Anti-USP14/TGT antibody (ab137432) at 1/1000 dilution + Rat brain cell lyaste at 50 µg

    Predicted band size: 56 kDa

    7.5 % SDS-PAGE
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling USP14/TGT with ab137432 at 1/500 dilution.


ab137432 has not yet been referenced specifically in any publications.

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