Overview

  • Product name

  • Description

    Rabbit polyclonal to USP14/TGT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human USP14/TGT aa 67-325.

  • Positive control

    • Human liver tissue; 293T, A431, Jurkat, Raji and Mouse brain cell lysates.
  • General notes

     This product was previously labelled as USP14

     

Properties

Applications

Our Abpromise guarantee covers the use of ab137433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).
  • Sequence similarities

    Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
    Contains 1 ubiquitin-like domain.
  • Cellular localization

    Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 14 antibody
    • TGT antibody
    • tRNA guanine transglycosylase 60 kD subunit antibody
    • tRNA guanine transglycosylase antibody
    • Ubiquitin carboxyl terminal hydrolase 14 antibody
    • Ubiquitin carboxyl-terminal hydrolase 14 antibody
    • Ubiquitin specific peptidase 14 antibody
    • Ubiquitin specific processing protease 14 antibody
    • Ubiquitin specific protease 14 antibody
    • Ubiquitin thiolesterase 14 antibody
    • Ubiquitin-specific-processing protease 14 antibody
    • UBP14_HUMAN antibody
    • USP 14 antibody
    • USP14 antibody
    see all

Images

  • All lanes : Anti-USP14/TGT antibody (ab137433) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : USP14/TGT Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 56 kDa

  • Anti-USP14/TGT antibody (ab137433) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 56 kDa



    7.5% SDS PAGE
  • Anti-USP14/TGT antibody (ab137433) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 56 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling USP14/TGT with ab137433 at 1/250 dilution.

References

This product has been referenced in:

  • Sha B  et al. Deubiquitylatinase inhibitor b-AP15 induces c-Myc-Noxa-mediated apoptosis in esophageal squamous cell carcinoma. Apoptosis 24:826-836 (2019). Read more (PubMed: 31342239) »
  • Teoh JP  et al. ß-arrestin-biased agonism of ß-adrenergic receptor regulates Dicer-mediated microRNA maturation to promote cardioprotective signaling. J Mol Cell Cardiol 118:225-236 (2018). WB, IP . Read more (PubMed: 29627294) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Egg extracts)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Egg extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 02 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up