• Product name

  • Description

    Rabbit polyclonal to USP14/TGT
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human USP14/TGT aa 67-325.

  • Positive control

    • Human liver tissue; 293T, A431, Jurkat, Raji and Mouse brain cell lysates.
  • General notes

     This product was previously labelled as USP14




Our Abpromise guarantee covers the use of ab137433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).


  • Function

    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).
  • Sequence similarities

    Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
    Contains 1 ubiquitin-like domain.
  • Cellular localization

    Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 14 antibody
    • TGT antibody
    • tRNA guanine transglycosylase 60 kD subunit antibody
    • tRNA guanine transglycosylase antibody
    • Ubiquitin carboxyl terminal hydrolase 14 antibody
    • Ubiquitin carboxyl-terminal hydrolase 14 antibody
    • Ubiquitin specific peptidase 14 antibody
    • Ubiquitin specific processing protease 14 antibody
    • Ubiquitin specific protease 14 antibody
    • Ubiquitin thiolesterase 14 antibody
    • Ubiquitin-specific-processing protease 14 antibody
    • UBP14_HUMAN antibody
    • USP 14 antibody
    • USP14 antibody
    see all


  • All lanes : Anti-USP14/TGT antibody (ab137433) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : USP14/TGT Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 56 kDa

  • Anti-USP14/TGT antibody (ab137433) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 56 kDa

    7.5% SDS PAGE
  • Anti-USP14/TGT antibody (ab137433) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 56 kDa

    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling USP14/TGT with ab137433 at 1/250 dilution.


This product has been referenced in:

  • Sha B  et al. Deubiquitylatinase inhibitor b-AP15 induces c-Myc-Noxa-mediated apoptosis in esophageal squamous cell carcinoma. Apoptosis 24:826-836 (2019). Read more (PubMed: 31342239) »
  • Teoh JP  et al. ß-arrestin-biased agonism of ß-adrenergic receptor regulates Dicer-mediated microRNA maturation to promote cardioprotective signaling. J Mol Cell Cardiol 118:225-236 (2018). WB, IP . Read more (PubMed: 29627294) »
See all 2 Publications for this product

Customer reviews and Q&As

Western blot
Xenopus laevis Cell lysate - whole cell (Egg extracts)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Egg extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

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Submitted Sep 02 2014

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