Product nameAnti-USP14/TGT antibody
See all USP14/TGT primary antibodies
DescriptionRabbit polyclonal to USP14/TGT
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
Synthetic peptide corresponding to Human USP14/TGT aa 350-400 (internal sequence).
Database link: NP_005142.1
- Whole cell lysates from Hela cells and 293T cells.
This product was previously labelled as USP14
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71165 in the following tested applications.
|WB||1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).
Sequence similaritiesBelongs to the peptidase C19 family. USP14/UBP6 subfamily.
Contains 1 ubiquitin-like domain.
Cellular localizationCytoplasm. Cell membrane.
- Information by UniProt
- Deubiquitinating enzyme 14 antibody
- TGT antibody
- tRNA guanine transglycosylase 60 kD subunit antibody
All lanes : Anti-USP14/TGT antibody (ab71165) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 28 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP14/TGT with ab71165 at 1/1000 (1µg/ml). Detection: DAB.
Detection of Human USP14/TGT by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab71165 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
ab71165 has been referenced in 1 publication.
- Chandrasekaran P et al. HIV-1 Nef down-modulates C-C and C-X-C chemokine receptors via ubiquitin and ubiquitin-independent mechanism. PLoS One 9:e86998 (2014). PubMed: 24489825