Recombinant
RabMAb

Recombinant Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

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Overview

  • Product name

    Anti-USP14/TGT antibody [EPR15943] - C-terminal
    See all USP14/TGT primary antibodies

  • Description

    Rabbit monoclonal [EPR15943] to USP14/TGT - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human USP14/TGT aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: P54578

  • Positive control

    • HCT-116, HeLa, Jurkat, 293T, C6 and RAW 264.7 cell lysates; HeLa and Jurkat cells.

  • General notes

     

     

     This product was previously labelled as USP14

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ICC/IF 1/70.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).

  • Sequence similarities

    Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
    Contains 1 ubiquitin-like domain.

  • Cellular localization

    Cytoplasm. Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Deubiquitinating enzyme 14 antibody
    • TGT antibody
    • tRNA guanine transglycosylase 60 kD subunit antibody
    • tRNA guanine transglycosylase antibody
    • Ubiquitin carboxyl terminal hydrolase 14 antibody
    • Ubiquitin carboxyl-terminal hydrolase 14 antibody
    • Ubiquitin specific peptidase 14 antibody
    • Ubiquitin specific processing protease 14 antibody
    • Ubiquitin specific protease 14 antibody
    • Ubiquitin thiolesterase 14 antibody
    • Ubiquitin-specific-processing protease 14 antibody
    • UBP14_HUMAN antibody
    • USP 14 antibody
    • USP14 antibody
    see all

Images

  • Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)This image is courtesy of an anonymous Abreview.
    All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/1000 dilution

    All lanes : HAP1 cell line

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : donkey anti-rabbit IgG-HRP at 1/3000 dilution

    Predicted band size: 56 kDa

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Immunocytochemistry/ Immunofluorescence - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

    Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling USP14/TGT with ab192618 at 1/70 dilution (5 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody.  Counterstain: DAPI.

  • Flow Cytometry - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Flow Cytometry - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

    Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling USP14/TGT with ab192618 at 1/70 dilution (red) or a rabbit IgG (negative) (black), unlabeled cells (blue) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HCT-116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 56 kDa

  • Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 56 kDa

References

This product has been referenced in:

  • Yu F  et al. Tumor suppressive microRNA-124a inhibits stemness and enhances gefitinib sensitivity of non-small cell lung cancer cells by targeting ubiquitin-specific protease 14. Cancer Lett 427:74-84 (2018). WB . Read more (PubMed: 29702194) »
  • Fletcher AJ  et al. Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling. Cell Host Microbe 24:761-775.e6 (2018). Read more (PubMed: 30503508) »
See all 2 Publications for this product

Publishing research using ab192618? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-USP14/TGT antibody [EPR15943] - C-terminal

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HAP1 cell line)
Gel Running Conditions
Non-reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
50 µg
Specification
HAP1 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

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Verified customer

Submitted Oct 10 2016

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