RecombinantRabMAb

Recombinant Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

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Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
  • Immunocytochemistry/ Immunofluorescence - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
  • Flow Cytometry - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
  • Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
  • Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
  • Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15943] to USP14/TGT - C-terminal
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-USP14/TGT antibody [EPR15943] - C-terminal
    See all USP14/TGT primary antibodies

  • Description

    Rabbit monoclonal [EPR15943] to USP14/TGT - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human USP14/TGT aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: P54578

  • Positive control

    • HCT-116, HeLa, Jurkat, 293T, C6 and RAW 264.7 cell lysates; HeLa and Jurkat cells.

  • General notes

     

     

     This product was previously labelled as USP14

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab192618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ICC/IF 1/70.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).

  • Sequence similarities

    Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
    Contains 1 ubiquitin-like domain.

  • Cellular localization

    Cytoplasm. Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Deubiquitinating enzyme 14 antibody
    • TGT antibody
    • tRNA guanine transglycosylase 60 kD subunit antibody
    • tRNA guanine transglycosylase antibody
    • Ubiquitin carboxyl terminal hydrolase 14 antibody
    • Ubiquitin carboxyl-terminal hydrolase 14 antibody
    • Ubiquitin specific peptidase 14 antibody
    • Ubiquitin specific processing protease 14 antibody
    • Ubiquitin specific protease 14 antibody
    • Ubiquitin thiolesterase 14 antibody
    • Ubiquitin-specific-processing protease 14 antibody
    • UBP14_HUMAN antibody
    • USP 14 antibody
    • USP14 antibody
    see all

Images

  • Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)This image is courtesy of an anonymous Abreview.
    All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/1000 dilution

    All lanes : HAP1 cell line

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : donkey anti-rabbit IgG-HRP at 1/3000 dilution

    Predicted band size: 56 kDa

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Immunocytochemistry/ Immunofluorescence - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

    Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling USP14/TGT with ab192618 at 1/70 dilution (5 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody.  Counterstain: DAPI.

  • Flow Cytometry - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Flow Cytometry - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

    Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling USP14/TGT with ab192618 at 1/70 dilution (red) or a rabbit IgG (negative) (black), unlabeled cells (blue) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HCT-116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 56 kDa

  • Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Western blot - Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 56 kDa

  • Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)
    Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618)

References (4)

Publishing research using ab192618? Please let us know so that we can cite the reference in this datasheet.

ab192618 has been referenced in 4 publications.

  • Zhang Y  et al. Lidocaine inhibits the proliferation and invasion of hepatocellular carcinoma by downregulating USP14 induced PI3K/Akt pathway. Pathol Res Pract N/A:152963 (2020). PubMed: 32471606
  • Xu J  et al. SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway. Cell Prolif 53:e12738 (2020). PubMed: 31957155
  • Yu F  et al. Tumor suppressive microRNA-124a inhibits stemness and enhances gefitinib sensitivity of non-small cell lung cancer cells by targeting ubiquitin-specific protease 14. Cancer Lett 427:74-84 (2018). WB . PubMed: 29702194
  • Fletcher AJ  et al. Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling. Cell Host Microbe 24:761-775.e6 (2018). PubMed: 30503508

Customer reviews and Q&As

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Western blot abreview for Anti-USP14/TGT antibody [EPR15943] - C-terminal

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HAP1 cell line)
Gel Running Conditions
Non-reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
50 µg
Specification
HAP1 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Oct 10 2016

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