Product nameAnti-USP15 antibody
See all USP15 primary antibodies
DescriptionGoat polyclonal to USP15
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig
General notesGene Ontology terms - ubiquitin-specific protease activity; cysteine-type endopeptidase activity; deubiquitination.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab4850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).|
|IHC-P||Use a concentration of 2.5 - 3.8 µg/ml.
Perform heat mediated antigen retrieval with citrate buffer (pH6).
FunctionProtease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
Tissue specificityExpressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 1 DUSP domain.
modificationsPhosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
Ubiquitinated, leading to degradation by the proteasome.
- Information by UniProt
- Deubiquitinating enzyme 15 antibody
- Deubiquitinating enzyme antibody
- EC 3 1 2 15 antibody
Immunohistochemical analysis of paraffin embedded Human Liver labeling USP15 with ab4850 at 2.5-3.8µg/ml. The image shows patterned intracellular staining in hepatocytes.
Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
All lanes : Anti-USP15 antibody (ab4850) at 1/1000 dilution
All lanes : 20µg mouse brain whole cell lysate
All lanes : Donkey anti-goat conjugated to HRP
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 112 kDa
ab4850 has not yet been referenced specifically in any publications.