Key features and details
- Rabbit polyclonal to USP15
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-USP15 antibody
See all USP15 primary antibodies
DescriptionRabbit polyclonal to USP15
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region within amino acids 941-952 of Human USP15 (NP_006304).
- WB: 293T, A431, H1299, HeLa, MOLT4 and Raji cell lines IHC-P: N87 xenograft
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 112 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/200.|
FunctionProtease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
Tissue specificityExpressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 1 DUSP domain.
modificationsPhosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
Ubiquitinated, leading to degradation by the proteasome.
- Information by UniProt
- Deubiquitinating enzyme 15 antibody
- Deubiquitinating enzyme antibody
- EC 3 1 2 15 antibody
Anti-USP15 antibody (ab97533) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 112 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed A431, using USP15(GTX104644) antibody at 1:200 dilution.
Immunohistochemical analysis of USP15 in paraffin embedded N87 xenograft, using ab97533 at a 1/100 dilution.
ab97533 has been referenced in 2 publications.
- Teyra J et al. Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15. Structure 27:590-605.e5 (2019). PubMed: 30713027
- Eichhorn PJ et al. USP15 stabilizes TGF-ß receptor I and promotes oncogenesis through the activation of TGF-ß signaling in glioblastoma. Nat Med 18:429-35 (2012). PubMed: 22344298