Key features and details
- Rabbit polyclonal to USP16
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-USP16 antibody
See all USP16 primary antibodies
DescriptionRabbit polyclonal to USP16
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
antigen sequence, corresponding to amino acids 104-229 of Human USP16.
- WB: Human liver lysate. IHC: Human gastrointestinal, pancreas, testis and kidney tissue ICC/IF: Human cell line U-2 OS
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 94 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSpecifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.
Tissue specificityPresent in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Involvement in diseaseNote=A chromosomal aberration involving USP16 is a cause of Chronic myelomonocytic leukemia. Inversion inv(21) (q21;q22) with RUNX1/AML1.
Sequence similaritiesBelongs to the peptidase C19 family. USP16 subfamily.
Contains 1 UBP-type zinc finger.
DomainThe UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.
modificationsPhosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. The phosphorylated form of the protein is also enzymatically active.
- Information by UniProt
- Deubiquitinating enzyme 16 antibody
- Human ubiquitin processing protease antibody
- MSTP039 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121650 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Anti-USP16 antibody (ab121650) + Human liver tissue lysate
Predicted band size: 94 kDa
ab121650, at 1/1000 dilution, staining USP16 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
ab121650, at 1/1000 dilution, staining USP16 in paraffin-embedded Human gastrointestinal tissue by Immunohistochemistry.
ab121650, at 1/1000 dilution, staining USP16 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
ab121650, at 1/1000 dilution, staining USP16 in paraffin-embedded Human testis tissue by Immunohistochemistry.
ab121650 has been referenced in 1 publication.
- Bianco K et al. Placental transcriptomes in the common aneuploidies reveal critical regions on the trisomic chromosomes and genome-wide effects. Prenat Diagn 36:812-22 (2016). WB, IHC-P . PubMed: 27328057