Recombinant Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20914-47] to USP16 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-USP16 antibody [EPR20914-47] - BSA and Azide free
See all USP16 primary antibodies -
Description
Rabbit monoclonal [EPR20914-47] to USP16 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat testis and liver lysates; Mouse testis lysate; MEF whole cell lysate.
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General notes
ab269961 is the carrier-free version of ab236628.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20914-47 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa). |
Target
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Function
Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. -
Tissue specificity
Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Involvement in disease
Note=A chromosomal aberration involving USP16 is a cause of Chronic myelomonocytic leukemia. Inversion inv(21) (q21;q22) with RUNX1/AML1. -
Sequence similarities
Belongs to the peptidase C19 family. USP16 subfamily.
Contains 1 UBP-type zinc finger. -
Domain
The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin. -
Post-translational
modificationsPhosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. The phosphorylated form of the protein is also enzymatically active. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 74112 Mouse
- Entrez Gene: 288306 Rat
- GenBank: NM_024258 Mouse
- SwissProt: Q99LG0 Mouse
- SwissProt: Q2KJ09 Rat
- Unigene: 152941 Mouse
- Unigene: 55800 Rat
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Alternative names
- Deubiquitinating enzyme 16 antibody
- Human ubiquitin processing protease antibody
- MSTP039 antibody
see all
Images
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All lanes : Anti-USP16 antibody [EPR20914-47] (ab236628) at 1/1000 dilution
Lane 1 : Rat testis tissue lysate at 20 µg
Lane 2 : Rat liver tissue lysate at 20 µg
Lane 3 : Mouse testis tissue lysate at 20 µg
Lane 4 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 94 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 26323689).
Exposure time: Lanes 1-3: 3 minutes Lane 4: 48 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab236628).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab269961 has not yet been referenced specifically in any publications.