Key features and details
- Mouse polyclonal to USP19
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-USP19 antibody
See all USP19 primary antibodies
DescriptionMouse polyclonal to USP19
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full-length Human protein (NP_006668.1, aa 1-1318).
- USP19-transfected 293T cells lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 175 kDa (predicted molecular weight: 145 kDa).|
FunctionDeubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates.
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 2 CS domains.
Contains 1 MYND-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 8430421I07Rik antibody
- AI047774 antibody
- Deubiquitinating enzyme 19 antibody
All lanes : Anti-USP19 antibody (ab68527) at 1/500 dilution
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 145 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
ab68527 has been referenced in 1 publication.
- Bernadotte A et al. In silico identification and biochemical characterization of the human dicarboxylate clamp TPR protein interaction network. FEBS Open Bio 8:1830-1843 (2018). PubMed: 30410862