Overview

  • Product name

    Anti-USP19 antibody [EPR14816]
    See all USP19 primary antibodies
  • Description

    Rabbit monoclonal [EPR14816] to USP19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human USP19 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: O94966

  • Positive control

    • Jurkat, 293T and HeLa lysates. Human fetal brain lysate. Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab189518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
Flow Cyt 1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates.
  • Sequence similarities

    Belongs to the peptidase C19 family.
    Contains 2 CS domains.
    Contains 1 MYND-type zinc finger.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 8430421I07Rik antibody
    • AI047774 antibody
    • Deubiquitinating enzyme 19 antibody
    • Ubiquitin carboxyl terminal hydrolase 19 antibody
    • Ubiquitin carboxyl-terminal hydrolase 19 antibody
    • Ubiquitin specific peptidase 19 antibody
    • Ubiquitin specific processing protease 19 antibody
    • Ubiquitin specific protease 19 antibody
    • Ubiquitin thioesterase 19 antibody
    • Ubiquitin thiolesterase 19 antibody
    • Ubiquitin-specific-processing protease 19 antibody
    • UBP19_HUMAN antibody
    • Usp19 antibody
    • Zinc finger MYND domain containing protein 9 antibody
    • Zinc finger MYND domain-containing protein 9 antibody
    • ZMYND9 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: USP19 knockout HAP1 cell lysate (40 µg)
    Lane 3: Jurkat cell lysate (40 µg)
    Lane 4: HeLa cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab189518 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab189518 was shown to recognize USP19  when USP19  knockout samples were used, along with additional cross-reactive bands. Wild-type and USP19  knockout samples were subjected to SDS-PAGE. Ab189518 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow cytometric analysis of Jurkat cells (left histogram) labeling USP19 with ab189518 at 1/250 dilution compared to negative control cells (right histogram). Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.

  • Anti-USP19 antibody [EPR14816] (ab189518) at 1/5000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 146 kDa

  • All lanes : Anti-USP19 antibody [EPR14816] (ab189518) at 1/10000 dilution

    Lane 1 : Jurkat lysate
    Lane 2 : 293T lysate
    Lane 3 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 146 kDa

References

ab189518 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HAP1 cell line)
Gel Running Conditions
Non-reduced Denaturing (4-12 % Bis-Tris gel)
Loading amount
50 µg
Specification
HAP1 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 10 2016

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