Overview

  • Product name

  • Description

    Rabbit polyclonal to USP20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human USP20 (internal sequence). The exact sequence is proprietary.
    Database link: Q9Y2K6

  • Positive control

    • WB: HEK-293T and Raji whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 102 kDa.

Target

  • Function

    Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Sequence similarities

    Belongs to the peptidase C19 family. USP20/USP33 subfamily.
    Contains 2 DUSP domains.
    Contains 1 UBP-type zinc finger.
  • Domain

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue.
  • Post-translational
    modifications

    Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
  • Cellular localization

    Cytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 20 antibody
    • hVDU2 antibody
    • KIAA1003 antibody
    • LSFR 3A antibody
    • LSFR3A antibody
    • pVHL interacting deubiquitinating enzyme 2 antibody
    • Ubiquitin carboxyl terminal hydrolase 20 antibody
    • Ubiquitin carboxyl-terminal hydrolase 20 antibody
    • ubiquitin specific peptidase 20 antibody
    • Ubiquitin specific processing protease 20 antibody
    • Ubiquitin specific protease 20 antibody
    • Ubiquitin thioesterase 20 antibody
    • Ubiquitin thiolesterase 20 antibody
    • Ubiquitin-specific-processing protease 20 antibody
    • UBP20_HUMAN antibody
    • USP 20 antibody
    • USP20 antibody
    • VDU 2 antibody
    • VDU2 antibody
    • VHL interacting deubiquitinating enzyme 2 antibody
    • VHL-interacting deubiquitinating enzyme 2 antibody
    see all

Images

  • All lanes : Anti-USP20 antibody (ab229781) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell extracts

    Lysates/proteins at 60 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 102 kDa



    7.5% SDS-PAGE

References

ab229781 has not yet been referenced specifically in any publications.

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