Key features and details
- Rabbit polyclonal to USP20
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-USP20 antibody
See all USP20 primary antibodies
DescriptionRabbit polyclonal to USP20
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human USP20 (internal sequence). The exact sequence is proprietary.
Database link: Q9Y2K6
- WB: HEK-293T and Raji whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab229781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 102 kDa.|
FunctionDeubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Sequence similaritiesBelongs to the peptidase C19 family. USP20/USP33 subfamily.
Contains 2 DUSP domains.
Contains 1 UBP-type zinc finger.
DomainThe UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue.
modificationsUbiquitinated via a VHL-dependent pathway for proteasomal degradation.
Cellular localizationCytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
- Information by UniProt
- Deubiquitinating enzyme 20 antibody
- hVDU2 antibody
- KIAA1003 antibody
All lanes : Anti-USP20 antibody (ab229781) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell extracts
Lysates/proteins at 60 µg per lane.
Developed using the ECL technique.
Predicted band size: 102 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229781 has not yet been referenced specifically in any publications.