• Product name

  • Description

    Rabbit polyclonal to USP20
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Chicken, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 863 and 913 of human USP20 (NP_006667.2).



Our Abpromise guarantee covers the use of ab72225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function

    Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Sequence similarities

    Belongs to the peptidase C19 family. USP20/USP33 subfamily.
    Contains 2 DUSP domains.
    Contains 1 UBP-type zinc finger.
  • Domain

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue.
  • Post-translational

    Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
  • Cellular localization

    Cytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 20 antibody
    • hVDU2 antibody
    • KIAA1003 antibody
    • LSFR 3A antibody
    • LSFR3A antibody
    • pVHL interacting deubiquitinating enzyme 2 antibody
    • Ubiquitin carboxyl terminal hydrolase 20 antibody
    • Ubiquitin carboxyl-terminal hydrolase 20 antibody
    • ubiquitin specific peptidase 20 antibody
    • Ubiquitin specific processing protease 20 antibody
    • Ubiquitin specific protease 20 antibody
    • Ubiquitin thioesterase 20 antibody
    • Ubiquitin thiolesterase 20 antibody
    • Ubiquitin-specific-processing protease 20 antibody
    • UBP20_HUMAN antibody
    • USP 20 antibody
    • USP20 antibody
    • VDU 2 antibody
    • VDU2 antibody
    • VHL interacting deubiquitinating enzyme 2 antibody
    • VHL-interacting deubiquitinating enzyme 2 antibody
    see all


  • All lanes : Anti-USP20 antibody (ab72225) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 102 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?

    Exposure time: 3 minutes
  • Detection of USP20 by Western Blot of Immunprecipitate.
    ab72225 at 1µg/ml staining USP20 in HeLa whole cell lysate immunoprecipitated using ab72225 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.


ab72225 has not yet been referenced specifically in any publications.

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