Overview

  • Product name
  • Description
    Rabbit polyclonal to USP21
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH selected from the C-terminal region of human USP21.

  • Positive control
    • Human breast carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab38864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 63 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates.
  • Tissue specificity
    Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.
  • Sequence similarities
    Belongs to the peptidase C19 family. USP21 subfamily.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 21 antibody
    • deubiquitinating enzyme 23 antibody
    • EC 3.1.2.15 antibody
    • ESTM28 antibody
    • MGC3394 antibody
    • NEDD8-specific protease antibody
    • Ubiquitin carboxyl terminal hydrolase 21 antibody
    • ubiquitin carboxyl terminal hydrolase 23 antibody
    • Ubiquitin carboxyl-terminal hydrolase 21 antibody
    • ubiquitin specific peptidase 21 antibody
    • Ubiquitin specific processing protease 21 antibody
    • ubiquitin specific protease 16 antibody
    • ubiquitin specific protease 21 antibody
    • ubiquitin specific protease 23 antibody
    • Ubiquitin thioesterase 21 antibody
    • Ubiquitin thiolesterase 21 antibody
    • ubiquitin thiolesterase 23 antibody
    • ubiquitin-specific protease 23, formerly antibody
    • Ubiquitin-specific-processing protease 21 antibody
    • UBP21_HUMAN antibody
    • USP16 antibody
    • Usp21 antibody
    • USP23 antibody
    • USP23, formerly antibody
    • W53272 antibody
    see all

Images

  • Anti-USP21 antibody (ab38864) + T47D cell lysate

    Predicted band size: 63 kDa

  • Formalin fixed and paraffin embedded human breast carcinoma tissue stained with ab38864, followed by AEC staining.

References

This product has been referenced in:
  • Liu Q  et al. Broad and diverse mechanisms used by deubiquitinase family members in regulating the type I interferon signaling pathway during antiviral responses. Sci Adv 4:eaar2824 (2018). Read more (PubMed: 29732405) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us.

Your credit note ID for the refundis xxx.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice.

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Answer

Thank you for your reply and for the additional information that will help us to reevaluate this antibody.

Yes, I'd be happy to compensate you with eithera credit (can be used on future Abcam purchase) or a refund (money back into your account).

Please let me know which one you would prefer, and I will arrange thatfor you.

I look forward to hear back from you and resolve this case.

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Answer

Thank you for your email. I am sorry to hear that this antibody is not providing satisfactory results.

Could you please provide the following additional details whichwill enable us to investigate this case and will provide us with vital information for monitoring product quality:

1) Where do you see bands on your WB? The protein can have 3 isoforms (63, 61 and 42 kDa). Do you see such bands?
2) Did the transfection work very well?
3) What antibody dilution and incubation time was used?
4) What blocking reagent was employed?
5) Is the secondary antibody working well otherwise?

I'd be happy tooffer youa refund. Other options would be a free of charge replacement with another antibody or a credit note. Please let me know whatyou would prefer.

I look forward to hear back from you and resolve this case.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
0.034 µg
Specification
Catalytic domain of USP21
Gel Running Conditions
Non-reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Human Purified protein (Catalytic domain of USP21)

Mr. Reggy Ekkebus

Verified customer

Submitted Jul 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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