Overview

  • Product name

  • Description

    Rabbit polyclonal to USP22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment within Human USP22 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UPT9

  • Positive control

    • WB: A549 whole cell lysate. IP: A549 whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab227523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
  • Tissue specificity

    Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
  • Sequence similarities

    Belongs to the peptidase C19 family. UBP8 subfamily.
    Contains 1 UBP-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 22 antibody
    • KIAA1063 antibody
    • Ubiquitin carboxyl terminal hydrolase 22 antibody
    • Ubiquitin carboxyl-terminal hydrolase 22 antibody
    • Ubiquitin specific peptidase 22 antibody
    • Ubiquitin specific peptidase 3 like antibody
    • Ubiquitin specific processing protease 22 antibody
    • Ubiquitin specific protease 22 antibody
    • Ubiquitin thioesterase 22 antibody
    • Ubiquitin thiolesterase 22 antibody
    • Ubiquitin-specific-processing protease 22 antibody
    • UBP22_HUMAN antibody
    • USP 22 antibody
    • Usp22 antibody
    • USP3L antibody
    see all

Images

  • Anti-USP22 antibody (ab227523) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS-PAGE gel.

  • USP22 was immunoprecipitated from A549 (human lung carcinoma cell line) whole cell extract with 5 µg of ab227523. Western blot was performed from the immunoprecipitate using ab227523.

    Lane 1: Control IgG instaed of ab227523 in A549 whole cell extract. 

    Lane 2: ab227523 IP in A549 whole cell extract. 

References

ab227523 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227523.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up