• Product name

    Anti-USP22 antibody [EPR4352(2)]
    See all USP22 primary antibodies
  • Description

    Rabbit monoclonal [EPR4352(2)] to USP22
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in human USP22

  • Positive control

    • HeLa, HAP1, human placenta and HT-1376 lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/1000 - 1/5000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
    • Tissue specificity

      Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
    • Sequence similarities

      Belongs to the peptidase C19 family. UBP8 subfamily.
      Contains 1 UBP-type zinc finger.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • Deubiquitinating enzyme 22 antibody
      • KIAA1063 antibody
      • Ubiquitin carboxyl terminal hydrolase 22 antibody
      • Ubiquitin carboxyl-terminal hydrolase 22 antibody
      • Ubiquitin specific peptidase 22 antibody
      • Ubiquitin specific peptidase 3 like antibody
      • Ubiquitin specific processing protease 22 antibody
      • Ubiquitin specific protease 22 antibody
      • Ubiquitin thioesterase 22 antibody
      • Ubiquitin thiolesterase 22 antibody
      • Ubiquitin-specific-processing protease 22 antibody
      • UBP22_HUMAN antibody
      • USP 22 antibody
      • Usp22 antibody
      • USP3L antibody
      see all


    • ab109435 (1/200) staining USP22 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

      See Abreview

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: USP22 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109435 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

      Ab109435 was shown to specifically react with USP22 in wild-type cells along with additional cross-reactive bands. The band was not seen in USP22 knockout HAP1 cells. Wild-type and USP22 knockout samples were subjected to SDS-PAGE. ab109435 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-USP22 antibody [EPR4352(2)] (ab109435) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : Human placenta lysate
      Lane 3 : HT-1376 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • Flow cytometric analysis of permeabilized HeLa cells using 1/10 ab109435 (red) or a rabbit IgG (negative) (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    ab109435 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A


    As requested, I have issued free of charge replacements.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More


    Thank you for following up your earlier complaints about these two antibodies, ab28664 and ab109435, with more details of your protocols and samples.

    I will be happy to replace them. We have not received other complaints about them but I suggest trying two different antibodies.

    For the RFP antibody, I suggest a mouse monoclonal, ab109809:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=109809).

    We unfortunately do not have an alternative to anti-USP22 ab109435, or even a different lot. If you would like an antibody against a different target instead, I can send that, or a credit or refund.

    I look forward to your reply.

    Read More
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)
    Yes - 0.5% Triton X100

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 16 2011

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