Overview

  • Product name

  • Description

    Rabbit polyclonal to USP24
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide from a region between residue 2410 and the C-terminus (residue 2460) of human USP24 (XP_371254.5)

  • Positive control

    • Whole cell lysate from Hela and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2,000 - 1/10,000. Detects bands of approximately 71 kDa and 250 kDa (predicted molecular weight: 211 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • All lanes : Anti-USP24 antibody (ab72241) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Predicted band size: 211 kDa
      Observed band size: 250 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands.

    • Immunoprecipitation/ Western Blot of USP24.
      Lane 1: ab72241 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72241 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 30 seconds.

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Question
    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    I apologize for the inconvenience. If you have purchased this product within 6 months, I would be pleased to offer you a free of charge replacementwith a new lot if available or offer a credit note/refund. In order top process this I would appreciate if you could provide the Abcam order confirmation number for this product.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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    Answer

    Thank you for contacting Abcam regarding our USP24 antibodies.


    Regarding the difference in molecular weight of the bands recognized by these antibodies:


    ab72241 - The WB image on our datasheet is representative of endogenous protein detection at the correct molecular weight.


    ab67579 - The WB image on our datasheet is representing detection of recombinant USP24 protein overexpressed in 293T cells. The plasmid does not contain a full length protein, but a fragment with a molecular weight of 37kDa. We expect the antibody to detect full length protein from endogenous samples at the predicted molecular weight.


    Regarding your IP experiment, I am not exactly sure why you detect two different bands in your lysate and your IP'd sample. In order to assist you further, would you please send me the details of you protocol and the samples you tested? Please also include an image if possible.


    I look forward to your reply so that I may assist you further. Please do not hesitate to contact me if you have any additional questions.

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    Answer

    Thank you for contacting us.

    I am very sorry to hear that you have been experiencing difficulties when using our products. In order to help me to better understand exactly what you are facing would you be able to send detailed protocols of your experiments using ab72241 and any images? Troubkleshooting steps you have already taken and controls you may have used would also be very helpful when trying to determine a way help you with this.

    I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

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