Product nameAnti-USP24 antibody
See all USP24 primary antibodies
DescriptionRabbit polyclonal to USP24
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide from a region between residue 2410 and the C-terminus (residue 2460) of human USP24 (XP_371254.5)
- Whole cell lysate from Hela and 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2,000 - 1/10,000. Detects bands of approximately 71 kDa and 250 kDa (predicted molecular weight: 211 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome.
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 1 UBA domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Deubiquitinating enzyme 24 antibody
- KIAA1057 antibody
- Ubiquitin carboxyl terminal hydrolase 24 antibody
All lanes : Anti-USP24 antibody (ab72241) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 211 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation/ Western Blot of USP24.
Lane 1: ab72241 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72241 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
This product has been referenced in:
- Luo H et al. WP1130 reveals USP24 as a novel target in T-cell acute lymphoblastic leukemia. Cancer Cell Int 19:56 (2019). Read more (PubMed: 30911287) »
- Wang K et al. Transcriptional regulation of human USP24 gene expression by NF-kappa B. J Neurochem 128:818-28 (2014). Read more (PubMed: 24286619) »