Overview

  • Product name

    Anti-USP24 antibody [EPR7060]
    See all USP24 primary antibodies
  • Description

    Rabbit monoclonal [EPR7060] to USP24
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human USP24 aa 2550-2650. The exact sequence is proprietary.

  • Positive control

    • Fetal heart, HeLa, A549 and 293T cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 290 kDa (predicted molecular weight: 294 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Involved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome.
    • Sequence similarities

      Belongs to the peptidase C19 family.
      Contains 1 UBA domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links

    • Alternative names

      • Deubiquitinating enzyme 24 antibody
      • KIAA1057 antibody
      • Ubiquitin carboxyl terminal hydrolase 24 antibody
      • Ubiquitin carboxyl-terminal hydrolase 24 antibody
      • Ubiquitin specific peptidase 24 antibody
      • Ubiquitin specific processing protease 24 antibody
      • Ubiquitin specific protease 24 antibody
      • Ubiquitin thioesterase 24 antibody
      • Ubiquitin thiolesterase 24 antibody
      • Ubiquitin-specific-processing protease 24 antibody
      • UBP24_HUMAN antibody
      • Usp24 antibody
      see all

    Images

    • All lanes : Anti-USP24 antibody [EPR7060] (ab129064) at 1/10000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 294 kDa
      Observed band size: 290 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab129064 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions
    Non-reduced Denaturing (4-12 % Bis-Tris gel)
    Loading amount
    50 µg
    Specification
    HAP1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Oct 10 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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