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    usp25-antibody-ab191703.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Deubiquitination
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Anti-USP25 antibody (ab191703)

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Western blot - Anti-USP25 antibody (ab191703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP25 antibody (ab191703)

Key features and details

  • Rabbit polyclonal to USP25
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-USP25 antibody
    See all USP25 primary antibodies
  • Description

    Rabbit polyclonal to USP25
  • Host species

    Rabbit
  • Specificity

    Multiple isoforms of USP25 are known to exist.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human USP25 (internal sequence). 17 amino acid peptide.
    Database link: Q9UHP3

  • Positive control

    • Huma brain tissue; mouse brain tissue lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)

Applications

Our Abpromise guarantee covers the use of ab191703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 122 kDa.

Target

  • Function

    Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
    The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
  • Tissue specificity

    Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
  • Sequence similarities

    Belongs to the peptidase C19 family.
    Contains 1 UBA-like domain.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • Post-translational
    modifications

    Acetylated.
    Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially by SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
    Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
    Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
  • Cellular localization

    Cytoplasm and Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development.
  • Target information above from: UniProt accession Q9UHP3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29761 Human
    • Entrez Gene: 30940 Mouse
    • Omim: 604736 Human
    • SwissProt: Q9UHP3 Human
    • SwissProt: P57080 Mouse
    • Unigene: 473370 Human
    • Unigene: 40986 Mouse
    • Alternative names

      • Deubiquitinating enzyme 25 antibody
      • Ubiquitin carboxyl-terminal hydrolase 25 antibody
      • Ubiquitin specific peptidase 25 antibody
      • ubiquitin specific protease 25 antibody
      • ubiquitin specific protease USP25 antibody
      • Ubiquitin thiolesterase 25 antibody
      • Ubiquitin-specific-processing protease 25 antibody
      • UBP25_HUMAN antibody
      • USP on chromosome 21 antibody
      • USP21 antibody
      • USP25 antibody
      see all

    Images

    • Western blot - Anti-USP25 antibody (ab191703)
      Western blot - Anti-USP25 antibody (ab191703)
      All lanes : Anti-USP25 antibody (ab191703) at 1 µg/ml

      Lane 1 : mouse brain tissue lysate
      Lane 2 : mouse brain tissue lysate with blocking peptide

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 122 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP25 antibody (ab191703)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP25 antibody (ab191703)

      Immunohistochemical analysis of human brain tissue labeling USP25 using ab191703 at 5 μg/ml.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab191703? Please let us know so that we can cite the reference in this datasheet.

    ab191703 has not yet been referenced specifically in any publications.

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