Anti-USP25 antibody (ab191703)
Key features and details
- Rabbit polyclonal to USP25
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-USP25 antibody
See all USP25 primary antibodies -
Description
Rabbit polyclonal to USP25 -
Host species
Rabbit -
Specificity
Multiple isoforms of USP25 are known to exist. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human USP25 (internal sequence). 17 amino acid peptide.
Database link: Q9UHP3 -
Positive control
- Huma brain tissue; mouse brain tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab191703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 122 kDa. |
Target
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Function
Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function. -
Tissue specificity
Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 UBA-like domain.
Contains 2 UIM (ubiquitin-interacting motif) repeats. -
Post-translational
modificationsAcetylated.
Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially by SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels. -
Cellular localization
Cytoplasm and Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development. - Information by UniProt
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Database links
- Entrez Gene: 29761 Human
- Entrez Gene: 30940 Mouse
- Omim: 604736 Human
- SwissProt: Q9UHP3 Human
- SwissProt: P57080 Mouse
- Unigene: 473370 Human
- Unigene: 40986 Mouse
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Alternative names
- Deubiquitinating enzyme 25 antibody
- Ubiquitin carboxyl-terminal hydrolase 25 antibody
- Ubiquitin specific peptidase 25 antibody
see all
Images
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Western blot - Anti-USP25 antibody (ab191703)All lanes : Anti-USP25 antibody (ab191703) at 1 µg/ml
Lane 1 : mouse brain tissue lysate
Lane 2 : mouse brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 122 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP25 antibody (ab191703)Immunohistochemical analysis of human brain tissue labeling USP25 using ab191703 at 5 μg/ml.
Protocols
Datasheets and documents
References (0)
ab191703 has not yet been referenced specifically in any publications.
