Overview

Properties

Applications

  • Application notes
    Tested in peptide ELISA.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
    WB: No signal obtained yet but low background observed in Human Kidney and Testis extracts at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
  • Target

    • Function
      Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
      The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
    • Tissue specificity
      Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
    • Sequence similarities
      Belongs to the peptidase C19 family.
      Contains 1 UBA-like domain.
      Contains 2 UIM (ubiquitin-interacting motif) repeats.
    • Post-translational
      modifications
      Acetylated.
      Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially by SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
      Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
      Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
    • Cellular localization
      Cytoplasm and Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 25 antibody
      • Ubiquitin carboxyl-terminal hydrolase 25 antibody
      • Ubiquitin specific peptidase 25 antibody
      • ubiquitin specific protease 25 antibody
      • ubiquitin specific protease USP25 antibody
      • Ubiquitin thiolesterase 25 antibody
      • Ubiquitin-specific-processing protease 25 antibody
      • UBP25_HUMAN antibody
      • USP on chromosome 21 antibody
      • USP21 antibody
      • USP25 antibody
      see all

    References

    ab5092 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for getting back to us with this very useful information. It would be more conclusive if you had used recombinant protein or compared this antibody to another characterized one. After careful consideration, we have decided to keep this antibody in our catalogue and are looking for more feedback from our customers who have used this particular product. However, for your compensation we can offer you a credit note or a refund. Please do let us know what you would prefer and confirm your Purchase Order Number or Abcam Order Number. Thank you for your time and your effort.

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    Answer

    Many thanks for the additional information. I think we now understand your experiment and are almost convinced that you have shown that this Fast-Track antibody does not work. However given the lack of published work on USP25, especially work using antibodies, we want to ask one final question. What apparent MW was the USP25 band found at in SDS-PAGE? We just want to be reassured that the USP25 you observe isn't an alternatively spliced or proteolytically cleaved form that doesn't contain the RIMLSLSRTPADGR epitope that we used in manufacturing this antibody.

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    Answer

    Thank you for your reply and your clarification. Could you please let me know if the other antibody can actually detect USP 25 under the same conditions? Otherwise, it might just be low sensitivity (mass spec can be very sensitive). We appreciate your comments very much and take your complaint very seriously. We are looking forward to hearing from you soon.

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    Answer

    Thank you for your enquiry. We would be very grateful if you can clarify the followings: 1. Did you immunoprecipitate with this antibody? 2. Are you saying it works in IP but not on Western blot application? 3. Or did you IP with another antibody, show the IP contained USP25, then find our antibody gave nothing on western? We would like to emphasize that this is a Fast-Track antibody and it has only been tested for ELISA. We are looking forward to hearing from you soon.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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