For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Thank you for your prompt response. IP was done with another Ab. USP 25 in the IP-ed material was detected via mass spec. We are quite confident that USP 25 is present in the sample, since the results were confirmed in two separate experiments. Together with the IPs, we also ran 30ug of Jurkat whole cell extract. We were not able to detect any signal with 1:100 dilution of the USP 25 Ab in both IP and whole cell extract samples Let me know if you have any other questions.
Asked on May 04 2004
Thank you for your reply and your clarification. Could you please let me know if the other antibody can actually detect USP 25 under the same conditions? Otherwise, it might just be low sensitivity (mass spec can be very sensitive). We appreciate your comments very much and take your complaint very seriously. We are looking forward to hearing from you soon.
Answered on May 06 2004