Overview

  • Product name

    Anti-USP25 antibody [EPR15019]
    See all USP25 primary antibodies
  • Description

    Rabbit monoclonal [EPR15019] to USP25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human USP25 aa 50-200. The exact sequence is proprietary.
    Database link: Q9UHP3

  • Positive control

    • 293, A673, Raw264.7, PC-12 and BxPC-3 cell lysate. A673 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
    The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
  • Tissue specificity

    Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
  • Sequence similarities

    Belongs to the peptidase C19 family.
    Contains 1 UBA-like domain.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • Post-translational
    modifications

    Acetylated.
    Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially by SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
    Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
    Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
  • Cellular localization

    Cytoplasm and Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 25 antibody
    • Ubiquitin carboxyl-terminal hydrolase 25 antibody
    • Ubiquitin specific peptidase 25 antibody
    • ubiquitin specific protease 25 antibody
    • ubiquitin specific protease USP25 antibody
    • Ubiquitin thiolesterase 25 antibody
    • Ubiquitin-specific-processing protease 25 antibody
    • UBP25_HUMAN antibody
    • USP on chromosome 21 antibody
    • USP21 antibody
    • USP25 antibody
    see all

Images

  • All lanes : Anti-USP25 antibody [EPR15019] (ab187156) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : A673 cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 122 kDa
    Observed band size: 122 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • All lanes : Anti-USP25 antibody [EPR15019] (ab187156) at 1/1000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 122 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Flow cytometry analysis of USP25 expression in 2% paraformaldehyde-fixed A673 cells using ab187156 at 1/60 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody.

References

This product has been referenced in:

  • Cheng H  et al. Inhibition of tankyrase by a novel small molecule significantly attenuates prostate cancer cell proliferation. Cancer Lett 443:80-90 (2019). Read more (PubMed: 30472184) »
  • Liu B  et al. A quaternary tetramer assembly inhibits the deubiquitinating activity of USP25. Nat Commun 9:4973 (2018). Read more (PubMed: 30478318) »
See all 3 Publications for this product

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