Anti-USP26 antibody (ab101650)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-USP26 antibody
See all USP26 primary antibodies -
Description
Rabbit polyclonal to USP26 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human USP26 aa 650-750 conjugated to keyhole limpet haemocyanin.
(Peptide available asab115930) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; HeLa Nuclear; HEK293.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab101650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. PubMed: 24922532 | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 24922532 | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 104 kDa). |
Target
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Function
Involved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome (By similarity). Deubiquinates the androgen receptor and regulates the androgen receptor signaling pathway. -
Sequence similarities
Belongs to the peptidase C19 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 83844 Human
- Omim: 300309 Human
- SwissProt: Q9BXU7 Human
- Unigene: 333137 Human
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Alternative names
- Deubiquitinating enzyme 26 antibody
- MGC120066 antibody
- MGC120067 antibody
see all
Images
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Western blot - Anti-USP26 antibody (ab101650)All lanes : Anti-USP26 antibody (ab101650) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
with immunising peptide at 1 µg/ml
Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with immunising peptide at 1 µg/ml
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-USP26 antibody (ab101650)ICC/IF image of ab101650 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101650 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Li L et al. USP26 promotes esophageal squamous cell carcinoma metastasis through stabilizing Snail. Cancer Lett 448:52-60 (2019). Read more (PubMed: 30763716) »
- Kit Leng Lui S et al. USP26 regulates TGF-ß signaling by deubiquitinating and stabilizing SMAD7. EMBO Rep 18:797-808 (2017). Read more (PubMed: 28381482) »
