Product nameAnti-USP26 antibody
See all USP26 primary antibodies
DescriptionRabbit polyclonal to USP26
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human USP26 aa 650-750 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: HeLa; HeLa Nuclear; HEK293.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 24922532|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24922532|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 104 kDa).|
FunctionInvolved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome (By similarity). Deubiquinates the androgen receptor and regulates the androgen receptor signaling pathway.
Sequence similaritiesBelongs to the peptidase C19 family.
- Information by UniProt
- Deubiquitinating enzyme 26 antibody
- MGC120066 antibody
- MGC120067 antibody
All lanes : Anti-USP26 antibody (ab101650) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
with immunising peptide at 1 µg/ml
Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with immunising peptide at 1 µg/ml
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
ICC/IF image of ab101650 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101650 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Li L et al. USP26 promotes esophageal squamous cell carcinoma metastasis through stabilizing Snail. Cancer Lett 448:52-60 (2019). Read more (PubMed: 30763716) »
- Kit Leng Lui S et al. USP26 regulates TGF-ß signaling by deubiquitinating and stabilizing SMAD7. EMBO Rep 18:797-808 (2017). Read more (PubMed: 28381482) »