Overview

  • Product name

  • Description

    Goat polyclonal to USP28
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: SVELKHYIQEDN with a Cysteine residue linker, corresponding to internal sequence amino acids 674-685 of Human USP28 (NP_065937.1).

  • Positive control

    • Human Testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab130351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. Preliminary experiments gave bands at approx 150kDa and 50kDa in Human Bone Marrow lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 122kDa according to NP_065937.1. Both detected bands were successfully blocked by incubation with the immunizing peptide.
IHC-P Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
  • Sequence similarities

    Belongs to the peptidase C19 family. USP28 subfamily.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
  • Post-translational
    modifications

    Degradaded upon nickel ion level or hypoxia exposure.
    Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
  • Cellular localization

    Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 28 antibody
    • KIAA1515 antibody
    • Ubiquitin carboxyl terminal hydrolase 28 antibody
    • Ubiquitin carboxyl-terminal hydrolase 28 antibody
    • ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
    • Ubiquitin specific peptidase 28 antibody
    • Ubiquitin specific processing protease 28 antibody
    • Ubiquitin specific protease 28 antibody
    • Ubiquitin thioesterase 28 antibody
    • Ubiquitin thiolesterase 28 antibody
    • Ubiquitin-specific-processing protease 28 antibody
    • UBP28_HUMAN antibody
    • USP 28 antibody
    • USP28 antibody
    • USP28 protein antibody
    see all

Images

  • ab130351, at 3.8 µg/ml staining USP28 in paraffin-embedded Human testis tissue by Immunohistochemistry, with Alkaline Phosphatase detection.

References

ab130351 has not yet been referenced specifically in any publications.

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