Product nameAnti-USP28 antibody
See all USP28 primary antibodies
DescriptionRabbit polyclonal to USP28
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human USP28 aa 19-400.
SSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQ DTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQA DGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVG NTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQEL QYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLE DAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPL QVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELS RFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYR
Database link: Q96RU2
- U2OS, NIH 3T3 and INS-1 cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab188240 in the following tested applications.
|WB||1/2000. Predicted molecular weight: 122 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionDeubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
Sequence similaritiesBelongs to the peptidase C19 family. USP28 subfamily.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
modificationsDegradaded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
Cellular localizationNucleus > nucleoplasm.
- Information by UniProt
- Deubiquitinating enzyme 28 antibody
- KIAA1515 antibody
- Ubiquitin carboxyl terminal hydrolase 28 antibody
All lanes : Anti-USP28 antibody (ab188240) at 1/2000 dilution
Lane 1 : Lysate from U2OS cells transfected with a control si
Lane 2 : Lysate from U2OS cells transfected with a USP28 si
Lane 3 : NIH 3T3 cell lysate
Lane 4 : INS-1 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 122 kDa
Western blot analysis (using ab188240) of immunoprecipitates from U2OS cell extracts
Lane 1: Input
Lane 2: immunoprecipitate with control serum+protein A sepharose.
Lane 3: immunoprecipitate with ab188240 + protein A sepharose.
Immunofluorescent analysis of 3% PFA-fixed U2OS cells transfected with either a control si (left) or USP28 si (right) labeling USP28 with ab188240 at 1/2000 dilution.
ab188240 has not yet been referenced specifically in any publications.