Key features and details
- Rabbit polyclonal to USP28
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-USP28 antibody
See all USP28 primary antibodies
DescriptionRabbit polyclonal to USP28
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Dog, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 50 and 100 of human USP28 (NP_065937.1).
- Whole cell lysates from 293T and HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 140, 100 kDa (predicted molecular weight: 122 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionDeubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
Sequence similaritiesBelongs to the peptidase C19 family. USP28 subfamily.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
modificationsDegradaded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
Cellular localizationNucleus > nucleoplasm.
- Information by UniProt
- Deubiquitinating enzyme 28 antibody
- KIAA1515 antibody
- Ubiquitin carboxyl terminal hydrolase 28 antibody
All lanes : Anti-USP28 antibody (ab70893) at 0.04 µg/ml
Lane 1 : Whole cell lysate from 293T cells at 50 µg
Lane 2 : Whole cell lysate from 293T cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 5 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Observed band size: 110,140 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP28 with ab70893 at 1/1000 (0.2µg/ml). Detection: DAB.
Detection of Human USP28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70893 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Note: bands between 55 and 71 kDa represent the IgG chains.
ab70893 has not yet been referenced specifically in any publications.