Anti-USP28 antibody - C-terminal (ab137438)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-USP28 antibody - C-terminal
See all USP28 primary antibodies -
Description
Rabbit polyclonal to USP28 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide, corresponding to a region within C terminal amino acids 1015-1077 of Human USP28.
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Positive control
- A431 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 122 kDa. |
Target
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Function
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. -
Sequence similarities
Belongs to the peptidase C19 family. USP28 subfamily.
Contains 1 UIM (ubiquitin-interacting motif) repeat. -
Post-translational
modificationsDegradaded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. -
Cellular localization
Nucleus > nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 57646 Human
- Entrez Gene: 235323 Mouse
- Entrez Gene: 315639 Rat
- Omim: 610748 Human
- SwissProt: Q96RU2 Human
- SwissProt: Q5I043 Mouse
- SwissProt: D3ZJ96 Rat
- Unigene: 503891 Human
see all -
Alternative names
- Deubiquitinating enzyme 28 antibody
- KIAA1515 antibody
- Ubiquitin carboxyl terminal hydrolase 28 antibody
see all
Images
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Western blot - Anti-USP28 antibody - C-terminal (ab137438)Anti-USP28 antibody - C-terminal (ab137438) at 1/500 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 122 kDa
7.5% SDS PAGE
Protocols
References
ab137438 has not yet been referenced specifically in any publications.
