Overview

  • Product name

    Anti-USP28 antibody - C-terminal
    See all USP28 primary antibodies
  • Description

    Rabbit polyclonal to USP28 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 1015-1077 of Human USP28.

  • Positive control

    • A431 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab137438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 122 kDa.

Target

  • Function

    Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
  • Sequence similarities

    Belongs to the peptidase C19 family. USP28 subfamily.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
  • Post-translational
    modifications

    Degradaded upon nickel ion level or hypoxia exposure.
    Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
  • Cellular localization

    Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 28 antibody
    • KIAA1515 antibody
    • Ubiquitin carboxyl terminal hydrolase 28 antibody
    • Ubiquitin carboxyl-terminal hydrolase 28 antibody
    • ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
    • Ubiquitin specific peptidase 28 antibody
    • Ubiquitin specific processing protease 28 antibody
    • Ubiquitin specific protease 28 antibody
    • Ubiquitin thioesterase 28 antibody
    • Ubiquitin thiolesterase 28 antibody
    • Ubiquitin-specific-processing protease 28 antibody
    • UBP28_HUMAN antibody
    • USP 28 antibody
    • USP28 antibody
    • USP28 protein antibody
    see all

Images

  • Anti-USP28 antibody - C-terminal (ab137438) at 1/500 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 122 kDa



    7.5% SDS PAGE

References

ab137438 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137438.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up