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  1. Link

    usp28-antibody-epr42502-ab110744.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Deubiquitination
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-USP28 antibody [EPR4250(2)] (ab110744)

  • Datasheet
Reviews (1) Submit a question References (2)

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Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
  • Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
  • Anti-USP28 antibody [EPR4250(2)] (ab110744)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4250(2)] to USP28
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human USP28 protein (ab163618)
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Anti-MED25 antibody (ab229319)

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Overview

  • Product name

    Anti-USP28 antibody [EPR4250(2)]
    See all USP28 primary antibodies
  • Description

    Rabbit monoclonal [EPR4250(2)] to USP28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, SW480, HeLa, and A431 cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4250(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

Associated products

  • Alternative Versions

    • Anti-USP28 antibody [EPR4250(2)] - BSA and Azide free (ab247937)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human USP28 protein (ab163618)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 122 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 122 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Target

  • Function

    Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
  • Sequence similarities

    Belongs to the peptidase C19 family. USP28 subfamily.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
  • Post-translational
    modifications

    Degradaded upon nickel ion level or hypoxia exposure.
    Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
  • Cellular localization

    Nucleus > nucleoplasm.
  • Target information above from: UniProt accession Q96RU2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 57646 Human
    • Entrez Gene: 235323 Mouse
    • Entrez Gene: 315639 Rat
    • Omim: 610748 Human
    • SwissProt: Q96RU2 Human
    • SwissProt: Q5I043 Mouse
    • SwissProt: D3ZJ96 Rat
    • Unigene: 503891 Human
    • Unigene: 21630 Mouse
    • Unigene: 6599 Rat
    see all
  • Alternative names

    • Deubiquitinating enzyme 28 antibody
    • KIAA1515 antibody
    • Ubiquitin carboxyl terminal hydrolase 28 antibody
    • Ubiquitin carboxyl-terminal hydrolase 28 antibody
    • ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
    • Ubiquitin specific peptidase 28 antibody
    • Ubiquitin specific processing protease 28 antibody
    • Ubiquitin specific protease 28 antibody
    • Ubiquitin thioesterase 28 antibody
    • Ubiquitin thiolesterase 28 antibody
    • Ubiquitin-specific-processing protease 28 antibody
    • UBP28_HUMAN antibody
    • USP 28 antibody
    • USP28 antibody
    • USP28 protein antibody
    see all

Images

  • Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
    Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: USP28 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab110744 observed at 128 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab110744 was shown to specifically react with USP28 when USP28 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP28 knockout samples were subjected to SDS-PAGE.  Ab110744 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
    Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
    All lanes : Anti-USP28 antibody [EPR4250(2)] (ab110744) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 122 kDa

  • Anti-USP28 antibody [EPR4250(2)] (ab110744)
    Anti-USP28 antibody [EPR4250(2)] (ab110744)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab110744? Please let us know so that we can cite the reference in this datasheet.

ab110744 has been referenced in 2 publications.

  • Tang M  et al. Antileukemic effect of caffeic acid 3,4-dihydroxyphenetyl ester. Evidences for its mechanisms of action. Phytomedicine 80:153383 (2021). PubMed: 33091855
  • Wrigley JD  et al. Identification and Characterization of Dual Inhibitors of the USP25/28 Deubiquitinating Enzyme Subfamily. ACS Chem Biol 12:3113-3125 (2017). PubMed: 29131570

Customer reviews and Q&As

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Western blot abreview for Anti-USP28 antibody [EPR4250(2)]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (egg extracts)
Specification
egg extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 02 2014

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