Recombinant Anti-USP28 antibody [EPR4250(2)] (ab110744)
![Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)](https://www.abcam.com/ps/products/110/ab110744/Images/ab110744-269759-anti-usp28-antibody-epr42502-western-blot.jpg "Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4250(2)] to USP28
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-USP28 antibody [EPR4250(2)]
See all USP28 primary antibodies -
Description
Rabbit monoclonal [EPR4250(2)] to USP28 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, SW480, HeLa, and A431 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4250(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 122 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 122 kDa. |
Target
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Function
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. -
Sequence similarities
Belongs to the peptidase C19 family. USP28 subfamily.
Contains 1 UIM (ubiquitin-interacting motif) repeat. -
Post-translational
modificationsDegradaded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. -
Cellular localization
Nucleus > nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 57646 Human
- Entrez Gene: 235323 Mouse
- Entrez Gene: 315639 Rat
- Omim: 610748 Human
- SwissProt: Q96RU2 Human
- SwissProt: Q5I043 Mouse
- SwissProt: D3ZJ96 Rat
- Unigene: 503891 Human
see all -
Alternative names
- Deubiquitinating enzyme 28 antibody
- KIAA1515 antibody
- Ubiquitin carboxyl terminal hydrolase 28 antibody
see all
Images
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Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: USP28 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab110744 observed at 128 kDa. Red - loading control, ab8245, observed at 37 kDa.ab110744 was shown to specifically react with USP28 when USP28 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP28 knockout samples were subjected to SDS-PAGE. Ab110744 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)](https://www.abcam.com/ps/products/110/ab110744/Images/ab110744-124036-anti-usp28-antibody-epr42502-western-blot.jpg)
Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)All lanes : Anti-USP28 antibody [EPR4250(2)] (ab110744) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 122 kDa -
![Anti-USP28 antibody [EPR4250(2)] (ab110744)](https://www.abcam.com/ps/products/110/ab110744/Images/ab110744-3-benefits-of-recombinant-antibodies.png)
Anti-USP28 antibody [EPR4250(2)] (ab110744)
Datasheets and documents
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Datasheet download
References (2)
ab110744 has been referenced in 2 publications.
- Tang M et al. Antileukemic effect of caffeic acid 3,4-dihydroxyphenetyl ester. Evidences for its mechanisms of action. Phytomedicine 80:153383 (2021). PubMed: 33091855
- Wrigley JD et al. Identification and Characterization of Dual Inhibitors of the USP25/28 Deubiquitinating Enzyme Subfamily. ACS Chem Biol 12:3113-3125 (2017). PubMed: 29131570
